PMID- 15720566
OWN - NLM
STAT- MEDLINE
DCOM- 20050411
LR  - 20111117
IS  - 0902-0055 (Print)
IS  - 0902-0055 (Linking)
VI  - 20
IP  - 2
DP  - 2005 Apr
TI  - Low salivary IgA activity to cell-surface antigens of mutans streptococci related 
      to HLA-DRB1*04.
PG  - 73-81
AB  - BACKGROUND/AIMS: Mutans streptococci are found in almost all individuals, though 
      there are large differences in colonization levels between individuals. These 
      differences are not readily explained, though several factors are believed to 
      influence the colonization. One factor is the immune response to mutans 
      streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous 
      study, differences in salivary IgA reactions to oral streptococci were observed 
      between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. 
      A lower salivary IgA activity to Streptococcus mutans in particular was most 
      pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this 
      study was to further investigate, in a larger study group, the salivary IgA 
      activity to antigens of three oral streptococci in relation to different 
      HLA-DRB1*04 alleles. METHODS: Stimulated saliva was collected from 58 
      HLA-DRB1*04-positive individuals. Whole cell antigen extracts from S. mutans, 
      Streptococcus sobrinus and Streptococcus parasanguis and the streptococcal 
      antigen (SA) I/II were separated in SDS-PAGE, transblotted and detected with 
      diluted saliva (Western blot), and analyzed in a computer program. All distinct 
      immunoblot bands over 100 kDa were recorded and compared in relation to DRB1*04. 
      RESULTS: The immunoblots revealed lower salivary IgA reactions to S. mutans, S. 
      sobrinus and SA I/II, but not to S. parasanguis, for the DRB1*0401- and 
      *0404-positive individuals compared to other DRB1*04 types. For the *0401 
      subgroup there was a significant association with a lower IgA response to S. 
      mutans. CONCLUSION: The results confirm earlier observations and may also support 
      previous demonstrated association between colonization by mutans streptococci and 
      the serologically defined HLA-DR4.
FAU - Wallengren, M L L
AU  - Wallengren ML
AD  - Department of Cariology, Faculty of Odontology, Malmo University, Malmo, Sweden. 
      marie-louise.wallengren@od.mah.se
FAU - Hamberg, K
AU  - Hamberg K
FAU - Ericson, D
AU  - Ericson D
FAU - Nordberg, J
AU  - Nordberg J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Oral Microbiol Immunol
JT  - Oral microbiology and immunology
JID - 8707451
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (HLA DRB1*04:04 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*04:01 antigen)
RN  - 0 (Immunoglobulin A, Secretory)
RN  - 0 (Salivary Proteins and Peptides)
RN  - 0 (wapA protein, Bacillus subtilis)
MH  - Adult
MH  - Aged
MH  - Antigens, Bacterial/*immunology
MH  - Bacterial Proteins/immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - HLA-DR Antigens/*immunology
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin A, Secretory/*immunology
MH  - Male
MH  - Middle Aged
MH  - Salivary Proteins and Peptides/*immunology
MH  - Streptococcus mutans/*immunology
MH  - Streptococcus sobrinus/immunology
EDAT- 2005/02/22 09:00
MHDA- 2005/04/12 09:00
CRDT- 2005/02/22 09:00
PHST- 2005/02/22 09:00 [pubmed]
PHST- 2005/04/12 09:00 [medline]
PHST- 2005/02/22 09:00 [entrez]
AID - OMI192 [pii]
AID - 10.1111/j.1399-302X.2004.00192.x [doi]
PST - ppublish
SO  - Oral Microbiol Immunol. 2005 Apr;20(2):73-81. doi: 
      10.1111/j.1399-302X.2004.00192.x.