PMID- 15720804
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20181113
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 6
IP  - 6
DP  - 2004 Nov-Dec
TI  - MMP-10 is overexpressed, proteolytically active, and a potential target for 
      therapeutic intervention in human lung carcinomas.
PG  - 777-85
AB  - Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix 
      is a major factor for tumor development and expansion. This study analysed MMP-10 
      protein expression and activity in human lung tumors of various grade, stage, and 
      type to address the relationship between MMP-10 and tumor characteristics and to 
      evaluate MMP-10 as a therapeutic target in non small cell lung carcinoma (NSCLC). 
      Unlike the majority of MMPs, MMP-10 was located in the tumor mass as opposed to 
      tumor stroma. MMP-10 protein was observed at low levels in normal human lung 
      tissues and at significantly higher levels in all types of NSCLC. No correlation 
      was observed between MMP-10 protein expression and tumor type, stage, or lymph 
      node invasion. To discriminate between active and inactive forms of MMP-10 in 
      samples of human NSCLC, we have developed an ex vivo fluorescent assay. 
      Measurable MMP-10 activity was detected in 42 of 50 specimens of lung cancer and 
      only 2 of 10 specimens of histologically normal lung tissue. No relationship was 
      observed between MMP-10 activity levels and clinicopathologic characteristics. 
      Our results suggest that MMP-10 is expressed and active at high levels in human 
      NSCLC compared to normal lung tissues, and, as such, is a potential target for 
      the development of novel therapeutics for lung cancer treatment.
FAU - Gill, Jason H
AU  - Gill JH
AD  - Cancer Research UK Laboratories, Tom Connors Cancer Research Centre, University 
      of Bradford, Bradford BD7 1DP, UK. j.gill1@bradford.ac.uk
FAU - Kirwan, Ian G
AU  - Kirwan IG
FAU - Seargent, Jill M
AU  - Seargent JM
FAU - Martin, Sandie W
AU  - Martin SW
FAU - Tijani, Sidiq
AU  - Tijani S
FAU - Anikin, Vladimir A
AU  - Anikin VA
FAU - Mearns, Alan J
AU  - Mearns AJ
FAU - Bibby, Michael C
AU  - Bibby MC
FAU - Anthoney, Alan
AU  - Anthoney A
FAU - Loadman, Paul M
AU  - Loadman PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (Biomarkers, Tumor)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.22 (Matrix Metalloproteinase 10)
SB  - IM
MH  - Animals
MH  - Biomarkers, Tumor/analysis
MH  - Carcinoma, Non-Small-Cell Lung/*enzymology/*pathology
MH  - Cell Line, Tumor
MH  - Enzyme Activation/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung Neoplasms/*enzymology/*pathology
MH  - Matrix Metalloproteinase 10
MH  - Metalloendopeptidases/*metabolism
MH  - Mice
MH  - Transplantation, Heterologous
PMC - PMC1550316
EDAT- 2005/02/22 09:00
MHDA- 2005/04/22 09:00
PMCR- 2004/11/01
CRDT- 2005/02/22 09:00
PHST- 2005/02/22 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2005/02/22 09:00 [entrez]
PHST- 2004/11/01 00:00 [pmc-release]
AID - 04283 [pii]
AID - 10.1593/neo.04283 [doi]
PST - ppublish
SO  - Neoplasia. 2004 Nov-Dec;6(6):777-85. doi: 10.1593/neo.04283.