PMID- 15720827
OWN - NLM
STAT- MEDLINE
DCOM- 20050719
LR  - 20051117
IS  - 1351-0002 (Print)
IS  - 1351-0002 (Linking)
VI  - 9
IP  - 6
DP  - 2004
TI  - Singlet oxygen quenching activity of human serum.
PG  - 325-30
AB  - Singlet oxygen is regarded as contributing to the pathogenesis of various 
      diseases including light-induced skin disorders and inflammatory response. In 
      this study, the correlation between singlet oxygen quenching activity (SOQA) of 
      human serum and blood biochemistry or life-style was evaluated. Healthy 
      volunteers were recruited and carried out a measurement of SOQA by using electron 
      paramagnetic resonance (EPR) and a questionnaire survey about a smoking. It was 
      demonstrated that major quenchers of singlet oxygen in serum are proteins, and 
      small molecular anti-oxidants relatively play a minor role. SOQA of whole sera 
      showed no correlation with protein concentration, but positively correlated with 
      SOQA of small molecular fraction. In vitro studies demonstrated that the decrease 
      of sulfhydryl groups by NO or superoxide significantly attenuated SOQA of 
      albumin. Together, these results may imply that the underlying oxidative 
      condition in each individual influences both small molecular antioxidant states 
      and the sulfhydryl content of serum proteins. SOQA of sera from women with a 
      smoking history was significantly lower compared to non-smoking women, suggesting 
      that the smoking habit impaired the defense mechanism against singlet oxygen.
FAU - Kon, Tatsuya
AU  - Kon T
AD  - Central Research Laboratory, FANCL Corporation, Kanagawa, Yokohama, Japan. 
      kon@fancl.co.jp
FAU - Tanigawa, Toru
AU  - Tanigawa T
FAU - Hayamizu, Kohsuke
AU  - Hayamizu K
FAU - Shen, Manzhen
AU  - Shen M
FAU - Tsuji, Tomoko
AU  - Tsuji T
FAU - Naito, Yuji
AU  - Naito Y
FAU - Yoshikawa, Toshikazu
AU  - Yoshikawa T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Redox Rep
JT  - Redox report : communications in free radical research
JID - 9511366
RN  - 0 (Blood Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (gamma-Globulins)
RN  - 17778-80-2 (Singlet Oxygen)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Blood Proteins/analysis/*physiology
MH  - Electron Spin Resonance Spectroscopy
MH  - Female
MH  - Humans
MH  - Life Style
MH  - Male
MH  - Middle Aged
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - *Serum
MH  - Serum Albumin/analysis
MH  - Singlet Oxygen/*blood/*physiology
MH  - Smoking/blood
MH  - Sulfhydryl Compounds/blood
MH  - gamma-Globulins/analysis
EDAT- 2005/02/22 09:00
MHDA- 2005/07/20 09:00
CRDT- 2005/02/22 09:00
PHST- 2005/02/22 09:00 [pubmed]
PHST- 2005/07/20 09:00 [medline]
PHST- 2005/02/22 09:00 [entrez]
AID - 10.1179/135100004225006821 [doi]
PST - ppublish
SO  - Redox Rep. 2004;9(6):325-30. doi: 10.1179/135100004225006821.