PMID- 15721198
OWN - NLM
STAT- MEDLINE
DCOM- 20050323
LR  - 20161124
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 43
IP  - 4
DP  - 2005 Apr
TI  - Characterization of antimutagenic mechanism of 3-allyl-5-substituted 
      2-thiohydantoins against 2-amino-3-methylimidazo[4,5-f]quinoline.
PG  - 521-8
AB  - 3-Allyl-5-substituted 2-thiohydantoins (ATH-amino acids) derived from allyl 
      isothiocyanate and amino acids can inhibit the mutagenicity of 
      2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in the Salmonella assay. In this 
      report, we studied possible mechanisms for the inhibition using rat liver S9 in 
      assays for ethoxyresorufin O-deethylase (EROD), a marker activity for cytochrome 
      P450 1A (CYP1A), which activates heterocyclic amines, and the Salmonella assays 
      with the direct-acting mutagen 2-hydroxyamino-3-methylimidazo[4,5-f]quinoline 
      (N-hydroxy-IQ). Quantitative analysis of ATH-amino acids and IQ during incubation 
      with rat liver S9 fraction by HPLC showed that ATH-amino acids could act as 
      S9-inhibitors, thereby inhibiting metabolic activation of IQ. Among the tested 
      ATH-amino acids, ATH-Phe, ATH-Trp, ATH-Leu and ATH-Val showed a dose-dependent 
      inhibition of EROD activity. ATH-Gly, ATH-Glu, and ATH-Asp behaved as blocking 
      agents toward N-hydroxy-IQ, but exhibited no inhibition of EROD activity.
FAU - Takahashi, Asaka
AU  - Takahashi A
AD  - Nagano Prefectural College, 8-49-7 Miwa, Nagano 380-8525, Japan. 
      asaka@nagano-kentan.ac.jp
FAU - Matsuoka, Hiroki
AU  - Matsuoka H
FAU - Yamada, Keiichi
AU  - Yamada K
FAU - Uda, Yasushi
AU  - Uda Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Biomarkers)
RN  - 0 (Mutagens)
RN  - 0 (Quinolines)
RN  - 0 (Thiohydantoins)
RN  - 30GL3D3T0G (2-amino-3-methylimidazo(4,5-f)quinoline)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cytochrome P-450 CYP1A1/analysis/pharmacology
MH  - DNA Damage
MH  - Dose-Response Relationship, Drug
MH  - Liver/enzymology
MH  - Mutagenicity Tests
MH  - Mutagens/*metabolism/*toxicity
MH  - Quinolines/*metabolism/*toxicity
MH  - Rats
MH  - Salmonella/genetics
MH  - Thiohydantoins/*pharmacology
EDAT- 2005/02/22 09:00
MHDA- 2005/03/24 09:00
CRDT- 2005/02/22 09:00
PHST- 2004/08/16 00:00 [received]
PHST- 2004/12/06 00:00 [revised]
PHST- 2004/12/12 00:00 [accepted]
PHST- 2005/02/22 09:00 [pubmed]
PHST- 2005/03/24 09:00 [medline]
PHST- 2005/02/22 09:00 [entrez]
AID - S0278-6915(05)00003-7 [pii]
AID - 10.1016/j.fct.2004.12.005 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2005 Apr;43(4):521-8. doi: 10.1016/j.fct.2004.12.005.