PMID- 1572136 OWN - NLM STAT- MEDLINE DCOM- 19920601 LR - 20190902 IS - 0732-8893 (Print) IS - 0732-8893 (Linking) VI - 15 IP - 2 DP - 1992 Feb TI - Comparison of enzyme-linked immunosorbent and indirect immunofluorescence assays for the detection of human T-cell lymphotropic virus type-I antibodies in sera from rural Haiti. PG - 121-4 AB - Serum samples were obtained from 340 healthy individuals without evidence of neurologic disease living in rural Haiti. Sera were screened for antibodies to human T-cell lymphotropic virus type I (HTLV-I) using two commercially available enzyme immunoassays (EIA) and by an indirect immunofluorescence assay (IFA) using a mixture of uninfected H9 cells and HTLV-I-infected MT-2 cells. Repeatedly positive samples were confirmed by Western blot (WB). Results with the two EIA systems were concordant and detected 13 positive samples, each of which was confirmed by WB. Only 9 (69%) of 13 WB-positive sera were detected by IFA, and four additional samples, positive by IFA, could not be confirmed by WB. The prevalence of HTLV-I seropositivity in this selected rural Haitian population was 3.8% (13 of 340). FAU - Grant, W AU - Grant W AD - Virology Reference Laboratory, VA Medical Center, West Haven, CT 06516. FAU - Bia, F J AU - Bia FJ FAU - Chacko, T M AU - Chacko TM FAU - Jean-Baptiste, M AU - Jean-Baptiste M FAU - Griffith, B P AU - Griffith BP LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Diagn Microbiol Infect Dis JT - Diagnostic microbiology and infectious disease JID - 8305899 RN - 0 (HTLV-I Antibodies) SB - IM MH - Adolescent MH - Adult MH - Aged MH - Blotting, Western MH - Cross Reactions MH - *Enzyme-Linked Immunosorbent Assay MH - Female MH - *Fluorescent Antibody Technique MH - HTLV-I Antibodies/*blood MH - HTLV-I Infections/*epidemiology MH - Haiti/epidemiology MH - Humans MH - Male MH - Middle Aged MH - Prevalence MH - Rural Population EDAT- 1992/02/01 00:00 MHDA- 1992/02/01 00:01 CRDT- 1992/02/01 00:00 PHST- 1992/02/01 00:00 [pubmed] PHST- 1992/02/01 00:01 [medline] PHST- 1992/02/01 00:00 [entrez] AID - 0732-8893(92)90034-Q [pii] AID - 10.1016/0732-8893(92)90034-q [doi] PST - ppublish SO - Diagn Microbiol Infect Dis. 1992 Feb;15(2):121-4. doi: 10.1016/0732-8893(92)90034-q.