PMID- 15722530
OWN - NLM
STAT- MEDLINE
DCOM- 20050411
LR  - 20200306
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 86
IP  - Pt 3
DP  - 2005 Mar
TI  - Phylogenetic analysis of human rhinovirus capsid protein VP1 and 2A protease 
      coding sequences confirms shared genus-like relationships with human 
      enteroviruses.
PG  - 697-706
LID - 10.1099/vir.0.80445-0 [doi]
AB  - Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human 
      rhinovirus (HRV) prototype strains revealed two major genetic clusters, similar 
      to that of the previously reported VP4/VP2 coding sequences, representing the 
      established two species, Human rhinovirus A (HRV-A) and Human rhinovirus B 
      (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 
      to 55 % between the two HRV species. Interserotypic sequence identities in both 
      HRV species were more variable than those within any Human enterovirus (HEV) 
      species in the same family. This means that unequivocal serotype identification 
      by VP1 sequence analysis used for HEV strains may not always be possible for HRV 
      isolates. On the other hand, a comprehensive insight into the relationships 
      between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like 
      situation. Distribution of pairwise nucleotide identity values between these 
      genera varied from 41 to 54 % in the VP1 coding region, similar to those between 
      heterologous members of the two HRV species. Alignment of the deduced amino acid 
      sequences revealed more fully conserved amino acid residues between HRV-B and 
      polioviruses than between the two HRV species. In phylogenetic trees, where all 
      HRVs and representatives from all HEV species were included, the two HRV species 
      did not cluster together but behaved like members of the same genus as the HEVs. 
      In conclusion, from a phylogenetic point of view, there are no good reasons to 
      keep these two human picornavirus genera taxonomically separated.
FAU - Laine, Pia
AU  - Laine P
AD  - Department of Microbiology, Enterovirus Laboratory, National Public Health 
      Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland.
FAU - Savolainen, Carita
AU  - Savolainen C
AD  - Department of Microbiology, Enterovirus Laboratory, National Public Health 
      Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland.
FAU - Blomqvist, Soile
AU  - Blomqvist S
AD  - Department of Microbiology, Enterovirus Laboratory, National Public Health 
      Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland.
FAU - Hovi, Tapani
AU  - Hovi T
AD  - Department of Microbiology, Enterovirus Laboratory, National Public Health 
      Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.29 (picornain 2A, Picornavirus)
SB  - IM
MH  - Cysteine Endopeptidases/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Enterovirus/classification/genetics
MH  - Humans
MH  - Phylogeny
MH  - Plant Proteins
MH  - Rhinovirus/*classification/genetics
MH  - Trans-Activators
MH  - Transcription Factors/*genetics
MH  - Viral Proteins/*genetics
EDAT- 2005/02/22 09:00
MHDA- 2005/04/12 09:00
CRDT- 2005/02/22 09:00
PHST- 2005/02/22 09:00 [pubmed]
PHST- 2005/04/12 09:00 [medline]
PHST- 2005/02/22 09:00 [entrez]
AID - 10.1099/vir.0.80445-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2005 Mar;86(Pt 3):697-706. doi: 10.1099/vir.0.80445-0.