PMID- 1572280
OWN - NLM
STAT- MEDLINE
DCOM- 19920603
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 130
IP  - 5
DP  - 1992 May
TI  - Production of various cytokines by normal human osteoblast-like cells in response 
      to interleukin-1 beta and tumor necrosis factor-alpha: lack of regulation by 17 
      beta-estradiol.
PG  - 2528-34
AB  - We investigated, in five cell strains per experiment, whether several cytokines 
      known or believed to have effects on bone resorption were produced by nearly 
      homogeneous strains of cultured normal human osteoblast-like (hOB) cells that 
      display virtually the complete phenotype of the mature osteoblast. In 
      unstimulated hOB cells, we detected constitutive production of interleukin-6 
      (IL-6) (mean +/- SE, 122 +/- 32 pg/ml) and IL-8 (135 +/- 39 pg/ml), but not of 
      IL-4, granulocyte-macrophage colony-stimulating factor (GM-CSF), or tumor 
      necrosis factor-alpha (TNF alpha). IL-1 beta in doses from 1-100 U/ml stimulated 
      dose-dependent increases in IL-6 (r = 0.87; P less than 0.001) and IL-8 (r = 
      0.95; P less than 0.001). Similar increases occurred after stimulation with TNF 
      alpha in doses from 3-300 U/ml. IL-1 beta and TNF alpha also stimulated GM-CSF 
      production, but only at higher doses. 17 beta-Estradiol (10(-8) M) had no 
      significant effect on the secretion of any of these cytokines, either 
      constitutively or after stimulation with IL-1 beta or TNF alpha. Stimulated 
      production of IL-4 was not detected after treatment with IL-1 beta or TNF alpha, 
      and that of TNF alpha was not detected after treatment with IL-1 beta. We 
      conclude that IL-6, IL-8, and GM-CSF, but not IL-4 and TNF alpha, are produced by 
      highly differentiated normal human cells of the osteoblast lineage, but their 
      secretion is not regulated by estrogen. However, we cannot exclude the 
      possibility that estrogen regulation of these cytokines may occur during early 
      stages of osteoblast differentiation.
FAU - Chaudhary, L R
AU  - Chaudhary LR
AD  - Division of Endocrinology and Metabolism, Mayo Clinic, Rochester, Minnesota 
      55905.
FAU - Spelsberg, T C
AU  - Spelsberg TC
FAU - Riggs, B L
AU  - Riggs BL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4TI98Z838E (Estradiol)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/*pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*biosynthesis
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Interleukin-6/*biosynthesis
MH  - Interleukin-8/*biosynthesis
MH  - Osteoblasts/drug effects/*metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Regression Analysis
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 10.1210/endo.130.5.1572280 [doi]
PST - ppublish
SO  - Endocrinology. 1992 May;130(5):2528-34. doi: 10.1210/endo.130.5.1572280.