PMID- 15723651 OWN - NLM STAT- MEDLINE DCOM- 20050407 LR - 20061115 IS - 1347-9032 (Print) IS - 1347-9032 (Linking) VI - 96 IP - 2 DP - 2005 Feb TI - Follicular lymphoma subgrouping by fluorescence in situ hybridization analysis. PG - 77-82 AB - The frequency of t(14;18) in follicular lymphoma (FL) in Japan has been reported to be low compared to North America and other European countries. Recently, it has also been reported that FL lacks t(14;18), mainly among histological grade 3b, and occasionally has a rearranged Bcl-6 gene. It is not known whether a difference in histology or immunostaining pattern exists between FL with and without t(14;18). We performed interphase fluorescence in situ hybridization (FISH) analysis to detect Bcl-2/IgH, Bcl-6 gene rearrangement, Bcl-2 gene amplification, and the cyclinD1/IgH gene in formalin-fixed paraffin embedded specimens from our FL archives. The correlation between morphological features, histological grades, immunohistochemical findings, and cytogenetical aberrations was studied. In total, we found that 28 of 47 cases (59.6%) had t(14;18). Bcl-6 gene rearrangement and extra Bcl-2 gene signals were found in five and two cases, respectively. Only one had cyclinD1/IgH fusion. Ten of 12 grade 1, nine of 17 grade 2, and 0 of two grade 3 cases had fusion signals, respectively. None of the above abnormalities were detected in 12 of 47 cases (25.5%). Our data confirmed a high frequency of t(14;18) in FL in grade 1, but a lower incidence among grade 2, that could be attributed to the lower incidence of the translocation in FL in Japan. Immunostaining of both Bcl-2 and CD10 was highly predictable for the presence of t(14;18); the positive predictive value was 75%, suggesting the usefulness of the staining. FAU - Sekiguchi, Naohiro AU - Sekiguchi N AD - Hematology Division, National Cancer Center Hospital, Tokyo, Japan. FAU - Kobayashi, Yukio AU - Kobayashi Y FAU - Yokota, Yukiko AU - Yokota Y FAU - Kusumoto, Shigeru AU - Kusumoto S FAU - Tanimoto, Kazuki AU - Tanimoto K FAU - Watanabe, Takashi AU - Watanabe T FAU - Matsuno, Yoshihiro AU - Matsuno Y FAU - Tobinai, Kensei AU - Tobinai K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Cancer Sci JT - Cancer science JID - 101168776 RN - 0 (Immunoglobulin Heavy Chains) SB - IM MH - Adult MH - Aged MH - *Chromosomes, Human, Pair 14 MH - *Chromosomes, Human, Pair 18 MH - Female MH - *Gene Rearrangement MH - *Genes, bcl-2 MH - Humans MH - Immunoglobulin Heavy Chains/analysis MH - Immunohistochemistry MH - In Situ Hybridization, Fluorescence MH - Lymphoma, Follicular/*genetics/metabolism/pathology MH - Male MH - Middle Aged MH - *Translocation, Genetic EDAT- 2005/02/23 09:00 MHDA- 2005/04/09 09:00 CRDT- 2005/02/23 09:00 PHST- 2005/02/23 09:00 [pubmed] PHST- 2005/04/09 09:00 [medline] PHST- 2005/02/23 09:00 [entrez] AID - CAS014 [pii] AID - 10.1111/j.1349-7006.2005.00014.x [doi] PST - ppublish SO - Cancer Sci. 2005 Feb;96(2):77-82. doi: 10.1111/j.1349-7006.2005.00014.x.