PMID- 15723970
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20220309
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 111
IP  - 8
DP  - 2005 Mar 1
TI  - Activation of hypoxia-inducible factor-1 in bacillary angiomatosis: evidence for 
      a role of hypoxia-inducible factor-1 in bacterial infections.
PG  - 1054-62
AB  - BACKGROUND: Bartonella species are the only known bacterial pathogens causing 
      vasculoproliferative disorders in humans (bacillary angiomatosis [BA]). Cellular 
      and bacterial pathogenetic mechanisms underlying the induction of BA are largely 
      unknown. METHODS AND RESULTS: Activation of hypoxia-inducible factor-1 (HIF-1), 
      the key transcription factor involved in angiogenesis, was detected in Bartonella 
      henselae-infected host cells in vitro by immunofluorescence, Western blotting, 
      electrophoretic mobility shift, and reporter gene assays and by 
      immunohistochemistry in BA tissue lesions in vivo. Gene microarray analysis 
      revealed that a B henselae infection resulted in the activation of genes typical 
      for the cellular response to hypoxia. HIF-1 was essential for B henselae-induced 
      expression of vascular endothelial growth factor as shown by inhibition with the 
      use of HIF-1-specific short-interfering RNA. Moreover, infection with B henselae 
      resulted in increased oxygen consumption, cellular hypoxia, and decreased ATP 
      levels in host cells. Infection with a pilus-negative variant of B henselae did 
      not lead to cellular hypoxia or activation of HIF-1 or vascular endothelial 
      growth factor secretion, suggesting a crucial role of this bacterial surface 
      protein in the angiogenic reprogramming of the host cells. CONCLUSIONS: B 
      henselae induces a proangiogenic host cell response via HIF-1. Our data provide 
      for the first time evidence that HIF-1 may play a role in bacterial infections.
FAU - Kempf, Volkhard A J
AU  - Kempf VA
AD  - Institut fur Medizinische Mikrobiologie und Hygiene, Eberhard-Karls Universitat, 
      Tubingen, Germany. volkhard.kempf@med.uni-tuebingen.de
FAU - Lebiedziejewski, Maria
AU  - Lebiedziejewski M
FAU - Alitalo, Kari
AU  - Alitalo K
FAU - Walzlein, Joo-Hee
AU  - Walzlein JH
FAU - Ehehalt, Urs
AU  - Ehehalt U
FAU - Fiebig, Jeannette
AU  - Fiebig J
FAU - Huber, Stephan
AU  - Huber S
FAU - Schutt, Burkhardt
AU  - Schutt B
FAU - Sander, Christian A
AU  - Sander CA
FAU - Muller, Steffen
AU  - Muller S
FAU - Grassl, Guntram
AU  - Grassl G
FAU - Yazdi, Amir S
AU  - Yazdi AS
FAU - Brehm, Bernhard
AU  - Brehm B
FAU - Autenrieth, Ingo B
AU  - Autenrieth IB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050221
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Angiomatosis, Bacillary/*pathology
MH  - Bartonella henselae/isolation & purification/pathogenicity
MH  - Cell Hypoxia/physiology
MH  - DNA-Binding Proteins/immunology/metabolism/*physiology
MH  - Endothelial Cells/microbiology
MH  - Endothelium, Vascular/cytology
MH  - Fimbriae, Bacterial/metabolism/physiology
MH  - HeLa Cells/chemistry/metabolism/microbiology
MH  - Histiocytes/chemistry/pathology
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Immunohistochemistry/methods
MH  - Macrophages/chemistry/pathology
MH  - Neovascularization, Pathologic/microbiology
MH  - Nuclear Proteins/immunology/metabolism/*physiology
MH  - Transcription Factors/immunology/metabolism/*physiology
MH  - Umbilical Veins/cytology
MH  - Up-Regulation/physiology
EDAT- 2005/02/23 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/02/23 09:00
PHST- 2005/02/23 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/02/23 09:00 [entrez]
AID - 01.CIR.0000155608.07691.B7 [pii]
AID - 10.1161/01.CIR.0000155608.07691.B7 [doi]
PST - ppublish
SO  - Circulation. 2005 Mar 1;111(8):1054-62. doi: 10.1161/01.CIR.0000155608.07691.B7. 
      Epub 2005 Feb 21.