PMID- 15724080
OWN - NLM
STAT- MEDLINE
DCOM- 20051003
LR  - 20190608
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 51
IP  - 3
DP  - 2005 Jun
TI  - Immunolocalization of steroidogenic enzymes P450scc, 3betaHSD, P450c17, and 
      P450arom in Gottingen miniature pig testes.
PG  - 299-304
AB  - The objective of this study was to investigate immunolocalization of 
      steroidogenic enzymes in Gottingen miniature (GM) pig testes. Testes of 6 adult 
      GM pigs were obtained in September 1996 (n=2), February (n=2) and June (n=2), 
      1997. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised 
      against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), 
      human placental 3beta-hydroxysteroid dehydrogenase (3betaHSD), porcine testicular 
      17alpha-hydroxylase cytochrome P450 (P450c17), and human placental aromatase 
      cytochrome P450 (P450arom). Histologically, all types of spermatogenic cells 
      including mature-phase spermatozoa in seminiferous tubules were observed in all 
      testes throughout the year. Moreover, P450scc, 3betaHSD, P450c17and P450arom were 
      identified in Leydig cells but not in Sertoli cells of all testes. These results 
      suggested that adult GM pig testes have the ability to produce germ cells 
      throughout the year, and the synthesis of progestin, androgen and estrogen occurs 
      in the Leydig cells of GM pig testes.
FAU - Weng, Qiang
AU  - Weng Q
AD  - Faculty of Biological Science and Technology, Beijing Forestry University, China.
FAU - Medan, Mohamed S
AU  - Medan MS
FAU - Watanabe, Gen
AU  - Watanabe G
FAU - Tsubota, Toshio
AU  - Tsubota T
FAU - Tanioka, Yoshikuni
AU  - Tanioka Y
FAU - Taya, Kazuyoshi
AU  - Taya K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050222
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/analysis
MH  - Animals
MH  - Aromatase/analysis
MH  - Cholesterol Side-Chain Cleavage Enzyme/analysis
MH  - Immunohistochemistry
MH  - Leydig Cells/chemistry/*enzymology
MH  - Male
MH  - Steroid 17-alpha-Hydroxylase/analysis
MH  - Swine
MH  - Swine, Miniature/anatomy & histology/*metabolism
MH  - Testis/chemistry/cytology/*enzymology
EDAT- 2005/02/23 09:00
MHDA- 2005/10/04 09:00
CRDT- 2005/02/23 09:00
PHST- 2005/02/23 09:00 [pubmed]
PHST- 2005/10/04 09:00 [medline]
PHST- 2005/02/23 09:00 [entrez]
AID - JST.JSTAGE/jrd/16077 [pii]
AID - 10.1262/jrd.16077 [doi]
PST - ppublish
SO  - J Reprod Dev. 2005 Jun;51(3):299-304. doi: 10.1262/jrd.16077. Epub 2005 Feb 22.