PMID- 15725600
OWN - NLM
STAT- MEDLINE
DCOM- 20050503
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 581
IP  - 1-2
DP  - 2005 Mar 7
TI  - Increased incidence of micronuclei assessed with the micronucleus assay and the 
      fluorescence in situ hybridization (FISH) technique in peripheral blood 
      lymphocytes of nurses exposed to nitrous oxide.
PG  - 1-9
AB  - It has been postulated that exposure to nitrous oxide and halogenated 
      anaesthetics is associated with various adverse health effects such as 
      neurological and reproductive abnormalities or impairment of hepatic functions. 
      In spite of the quite well known genotoxic effects of exposure to nitrous oxide 
      in vivo, the mechanisms of these effects are still not clear. The aim of this 
      study was to assess the frequency of micronuclei and to identify the type of 
      chromosomal damage (clastogenic or aneugenic) in peripheral blood lymphocytes of 
      operating-room nurses exposed to nitrous oxide. The study group comprised 46 
      women working at departments where the concentration of nitrous oxide ranged from 
      14 to 2308 mg/m3. The control population was composed of 28 women employed in the 
      same hospitals but in non-surgical departments. The clastogenic/aneugenic effect 
      of nitrous oxide was evaluated in lymphocytes using the standard micronucleus 
      (MN) assay in combination with the fluorescence in situ hybridization (FISH) 
      technique with pancentromeric probes. The results show a significant increase of 
      the MN frequency in lymphocytes of exposed nurses compared with the control group 
      (4.36+/-2.23 versus 9.02+/-4.67). The multiple regression analysis revealed a 
      statistically significant relationship (p=0.0009) between MN frequency and 
      exposure status, indicating that the level of exposure was the main factor 
      affecting chromosomal damage. As assessed by FISH analysis, the overall 
      frequencies of centromere-positive MN in the control and exposed groups were 43 
      and 49%, respectively. The increase observed in the exposed group may suggest a 
      slight, statistically insignificant pro-aneugenic effect of exposure to nitrous 
      oxide.
FAU - Lewinska, D
AU  - Lewinska D
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, 8 Teresy St., 90-950 Lodz, Poland. dobrysia@imp.lodz.pl
FAU - Stepnik, M
AU  - Stepnik M
FAU - Krajewski, W
AU  - Krajewski W
FAU - Arkusz, J
AU  - Arkusz J
FAU - Stanczyk, M
AU  - Stanczyk M
FAU - Wronska-Nofer, T
AU  - Wronska-Nofer T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Anesthetics, Inhalation)
RN  - K50XQU1029 (Nitrous Oxide)
SB  - IM
MH  - Adult
MH  - Anesthetics, Inhalation/*toxicity
MH  - Chromosomes, Human/*drug effects
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/cytology/*physiology
MH  - *Micronuclei, Chromosome-Defective
MH  - Micronucleus Tests
MH  - Middle Aged
MH  - Nitrous Oxide/*toxicity
MH  - *Nurses
MH  - Occupational Exposure
MH  - Statistics as Topic
EDAT- 2005/02/24 09:00
MHDA- 2005/05/04 09:00
CRDT- 2005/02/24 09:00
PHST- 2004/05/24 00:00 [received]
PHST- 2004/09/09 00:00 [revised]
PHST- 2004/10/10 00:00 [accepted]
PHST- 2005/02/24 09:00 [pubmed]
PHST- 2005/05/04 09:00 [medline]
PHST- 2005/02/24 09:00 [entrez]
AID - S1383-5718(04)00325-0 [pii]
AID - 10.1016/j.mrgentox.2004.10.018 [doi]
PST - ppublish
SO  - Mutat Res. 2005 Mar 7;581(1-2):1-9. doi: 10.1016/j.mrgentox.2004.10.018.