PMID- 15727153
OWN - NLM
STAT- MEDLINE
DCOM- 20050526
LR  - 20191109
IS  - 0923-9820 (Print)
IS  - 0923-9820 (Linking)
VI  - 16
IP  - 1
DP  - 2005 Feb
TI  - Conversion of food waste into hydrogen by thermophilic acidogenesis.
PG  - 33-44
AB  - Conversion of food waste into hydrogen by thermophilic acidogenesis was 
      investigated as a function of organic loading rate (OLR), hydraulic retention 
      time (HRT) and pH in a continuous stirred tank reactor. In order to identify 
      hydrogen-producing microorganisms, denaturing gradient gel electrophoresis (DGGE) 
      of the polymerase chain reaction (PCR)--amplified V3 region of 16S rDNA analysis 
      was conducted at each tested pH. The conversion of food waste into hydrogen was 
      strongly influenced by the operational conditions. The hydrogen production was 
      increased as OLR increased up to 8 gVSl(-1) d(-1), but drastically decreased at 
      10 gVSl(-1) d(-1). The yield of hydrogen was decreased from 2.2 to 1.0 
      mol-H2/mol-hexose consumed as HRT decreased from 5 to 2 days. More carbohydrates 
      in the food waste were decomposed at longer HRT, 76-90%, at HRT of 2-5 days. The 
      hydrogen production peaked at pH 5.5+/-0.1 and significantly decreased at pH 
      5.0+/-0.1. The biogas produced was composed of hydrogen and carbon dioxide, but 
      no methane was detected at all tested conditions. The hydrogen contents in the 
      gas produced were more than 55% (v/v) and not sensitive to all tested conditions. 
      The optimum operational condition for continuous hydrogen production from the 
      food waste was obtained at 8 gVSl(-1)d(-1), 5 days HRT and pH 5.5+/-0.1 where the 
      hydrogen production rate, content, yield and the efficiency of carbohydrate 
      decomposition were 1.01 H2/l-d, 60.5% (v/v), 2.2 mol-H2/mol-hexose consumed and 
      90%, respectively. The hydrogen production was related with the concentration of 
      total organic acids (TOA) which was strongly dependent on that of butyrate 
      indicating that the reaction was mainly butyrate fermentation. The 
      hydrogen-producing microorganism of Thermoanaerobacterium thermosaccharolyticum 
      that involved in acetate/butyrate fermentation, was detected with strong 
      intensity at all tested pHs by denaturing gradient gel electrophoresis (DGGE) of 
      the polymerase chain reaction (PCR)--amplified V3 region of 16S rDNA analysis and 
      sensitive to the tested pHs. The experimental results indicated that effective 
      hydrogen production from the food waste could be obtained continuously by 
      thermophilic acidogenesis at proper operational condition.
FAU - Shin, Hang-Sik
AU  - Shin HS
AD  - Department of Civil and Environmental Engineering, Korea Advanced Institute of 
      Science and Technology, Yuseong-gu, Daejeon 305-701, Korea.
FAU - Youn, Jong-Ho
AU  - Youn JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biodegradation
JT  - Biodegradation
JID - 9100834
RN  - 0 (Acids)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Acids/*metabolism
MH  - Biodegradation, Environmental
MH  - *Food
MH  - Hydrogen/*metabolism
MH  - Thermoanaerobacterium/*metabolism
EDAT- 2005/02/25 09:00
MHDA- 2005/05/27 09:00
CRDT- 2005/02/25 09:00
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/05/27 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - 10.1007/s10531-004-0377-9 [doi]
PST - ppublish
SO  - Biodegradation. 2005 Feb;16(1):33-44. doi: 10.1007/s10531-004-0377-9.