PMID- 15727255
OWN - NLM
STAT- MEDLINE
DCOM- 20050401
LR  - 20111117
IS  - 1090-6576 (Print)
IS  - 1090-6576 (Linking)
VI  - 8
IP  - 3
DP  - 2004 Fall
TI  - Five-locus HLA typing of hematopoietic stem cell donor volunteers using PCR 
      sequence specific primers.
PG  - 301-12
AB  - We have developed a strategy for five-locus human leukocyte antigen (HLA) typing 
      of hematopoeitic stem cell (HSC) donors using the polymerase chain reaction with 
      sequence-specific primers (PCR-SSP). The PCR-SSP method is robust, reproducible, 
      and accurate. New PCR-SSP mixtures can be added as required and all reactions are 
      carried out under the same conditions, which can easily be applied to the typing 
      of other loci, e.g., ABO blood groups. Initially, 127 PCR-SSP reactions were used 
      to detect simultaneously HLA-A, -B, -C, -DRB1/3/4/5, and DQB1 alleles, 
      differentiated generally to the level of the first two digits of the allele name, 
      essentially equivalent to the serological split specificity. Approximately 40% of 
      subjects were tested against a further 29 HLA-A, -B SSP mixtures to exclude rare 
      alleles and unambiguously assign a two-digit HLA allele family. This gave an 
      overall typing resolution equivalent to or greater than the split specificity 
      level and covered all HLA-A, -B, -C, -DRBland DQB1 alleles listed in the WHO's 
      Nomenclature for Factors of the HLA System, 2000. The Welsh Bone Marrow Donor 
      Registry has used this strategy to HLA type over 35,000 HSC donors over 9 years. 
      Comprehensive and accurate five-locus HLA typing allows confident and rapid 
      identification of potential matched HSC donors for patients requiring stem cell 
      transplantation generally without the need for typing additional loci. This 
      allows resources to be focused directly on allele level typing of DRB1 and other 
      loci. This strategy decreases overall donor work-up time, which is a major 
      benefit to patients.
FAU - Downing, J
AU  - Downing J
AD  - Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, 
      Pontyclun, Cardiff, Wales, UK. jonathan.downing@wbs.wales.nhs.uk
FAU - Guttridge, M G
AU  - Guttridge MG
FAU - Thompson, J
AU  - Thompson J
FAU - Darke, C
AU  - Darke C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genet Test
JT  - Genetic testing
JID - 9802546
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes, HLA)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA Probes, HLA/*chemistry
MH  - Female
MH  - Genotype
MH  - HLA Antigens/genetics
MH  - HLA-DR Antigens/genetics
MH  - HLA-DRB1 Chains
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - *Polymerase Chain Reaction
MH  - Sequence Analysis, DNA/methods
MH  - *Tissue Donors
EDAT- 2005/02/25 09:00
MHDA- 2005/04/02 09:00
CRDT- 2005/02/25 09:00
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/04/02 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - 10.1089/gte.2004.8.301 [doi]
PST - ppublish
SO  - Genet Test. 2004 Fall;8(3):301-12. doi: 10.1089/gte.2004.8.301.