PMID- 15727288 OWN - NLM STAT- MEDLINE DCOM- 20050323 LR - 20190917 IS - 0165-7380 (Print) IS - 0165-7380 (Linking) VI - 29 IP - 1 DP - 2005 Jan TI - Changes in activities of enzymes related to energy metabolism in peripheral leukocytes of fattening steers. PG - 19-26 AB - Glucose, triglyceride, cholesterol and immunoreactive insulin (IRI) concentrations, some enzyme activities in plasma, and activities of enzymes related to energy metabolism in peripheral leukocytes were measured in fattening Japanese Black Wagyu x Holstein steers fed on different diets at 8, 12, 16, 20 and 24 months of age. The plasma IRI concentrations at 20 and 24 months of age were significantly higher than those at 8 months of age. Activities of hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), aspartate aminotransferase (AST), and malate dehydrogenase (MDH) in cytosolic fractions, and glutamate dehydrogenase (GLDH), MDH and AST in mitochondrial fractions in peripheral leukocytes of steers at 24 months of age were significantly higher than those at 8 months. Increasing plasma insulin concentration was considered to induce acceleration of glucose utilization in leukocytes of fattening steers. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity in leukocytes increased significantly in the fattening process and was considered to be a useful indicator for evaluating changes in energy metabolism in steers. FAU - Kimura, N AU - Kimura N AD - Division of Animal Nutrition, Department of Animal Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. FAU - Yoshimura, I AU - Yoshimura I FAU - Sako, T AU - Sako T FAU - Inoue, A AU - Inoue A FAU - Tadami, K AU - Tadami K FAU - Arai, T AU - Arai T LA - eng PT - Journal Article PL - Switzerland TA - Vet Res Commun JT - Veterinary research communications JID - 8100520 RN - 0 (Blood Glucose) RN - 0 (Triglycerides) RN - 97C5T2UQ7J (Cholesterol) RN - EC 1.1.1.27 (L-Lactate Dehydrogenase) RN - EC 1.1.1.37 (Malate Dehydrogenase) SB - IM MH - Animals MH - Blood Glucose MH - Cattle/*blood MH - Cholesterol/blood MH - Energy Metabolism/*physiology MH - L-Lactate Dehydrogenase/blood MH - Leukocytes/*enzymology MH - Malate Dehydrogenase/blood MH - Male MH - Triglycerides/blood EDAT- 2005/02/25 09:00 MHDA- 2005/03/24 09:00 CRDT- 2005/02/25 09:00 PHST- 2005/02/25 09:00 [pubmed] PHST- 2005/03/24 09:00 [medline] PHST- 2005/02/25 09:00 [entrez] AID - 10.1023/b:verc.0000046735.40975.6e [doi] PST - ppublish SO - Vet Res Commun. 2005 Jan;29(1):19-26. doi: 10.1023/b:verc.0000046735.40975.6e.