PMID- 15727564
OWN - NLM
STAT- MEDLINE
DCOM- 20050609
LR  - 20050224
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 53
IP  - 3
DP  - 2005 Mar
TI  - Tumor necrosis factor-alpha in the placenta is not elevated in pre-eclamptic 
      patients despite its elevation in peripheral blood.
PG  - 113-9
AB  - PROBLEM: Tumor necrosis factor-alpha (TNF-alpha) is present in human placental 
      and uterine cells at the early and late stages of gestation and promotes the 
      regulation of trophoblast growth and invasion. We evaluated whether TNF-alpha 
      levels in the placenta and blood of pre-eclamptic women differed from those with 
      normal pregnancies. METHOD OF STUDY: The subjects were 39 pregnant women carrying 
      single fetuses (21 normal-pregnant and 18 pre-eclamptic patients). Their average 
      gestational age at entry was 38-39 weeks. Peripheral blood was collected before 
      the onset of labor and separated serum was stored at -20 degrees C. A tissue 
      segment of the placenta was cut and frozen in liquid nitrogen immediately after 
      delivery at -80 degrees C. The frozen placental tissue was added to 
      phosphate-buffered saline. The tissue was fully homogenized and centrifuged. 
      Separated supernatant was stored at -80 degrees C. TNF-alpha levels in separated 
      serum and TNF-alpha and total protein (TP) levels in separated supernatant were 
      measured. The presence of TNF-alpha in the placenta was evaluated by 
      immunohistochemistry in five pre-eclamptic and five normal-pregnant patients. 
      RESULTS: Serum TNF-alpha levels were higher in pre-eclampsia than in normal 
      pregnancies. However, TNF-alpha/TP levels in the placenta did not differ 
      significantly between the two groups. As for TNF-alpha immunostaining of 
      trophoblastic cells in the placenta, it was weak in three and moderate in two of 
      the normal pregnancies, while it was absent in two, weak in one, and moderate in 
      two in the pre-eclampsia group. CONCLUSIONS: We demonstrated no significant 
      increase in TNF-alpha/TP levels in the placenta in pre-eclampsia despite a 
      significant increase in serum TNF-alpha levels. There was no strong 
      immunostaining for TNF-alpha detected by immunohistochemistry in the 
      pre-eclampsia group. These findings suggest that TNF-alpha in the placenta is not 
      a key cytokine to interfere with normal trophoblast invasion into the myometrium 
      in pre-eclampsia, and that sources other than the placenta may contribute to the 
      elevated levels of TNF-alpha found in the circulation of pre-eclamptic patients.
FAU - Hayashi, Masatoshi
AU  - Hayashi M
AD  - Department of Obstetrics and Gynecology, Koshigaya Hospital, Dokkyo University 
      School of Medicine, Koshigaya-shi, Saitama, Japan. mhayashi@lilac.plala.or.jp
FAU - Ueda, Yoshihiko
AU  - Ueda Y
FAU - Yamaguchi, Takehiko
AU  - Yamaguchi T
FAU - Sohma, Ryoichi
AU  - Sohma R
FAU - Shibazaki, Mitsuei
AU  - Shibazaki M
FAU - Ohkura, Takeyoshi
AU  - Ohkura T
FAU - Inaba, Noriyuki
AU  - Inaba N
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Blood Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Birth Weight
MH  - Blood Pressure
MH  - Blood Proteins/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant, Newborn
MH  - Placenta/*metabolism
MH  - Pre-Eclampsia/*metabolism
MH  - Pregnancy
MH  - Regression Analysis
MH  - Serum/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2005/02/25 09:00
MHDA- 2005/06/10 09:00
CRDT- 2005/02/25 09:00
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/06/10 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - AJI253 [pii]
AID - 10.1111/j.1600-0897.2005.00253.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2005 Mar;53(3):113-9. doi: 10.1111/j.1600-0897.2005.00253.x.