PMID- 15727584
OWN - NLM
STAT- MEDLINE
DCOM- 20050620
LR  - 20121115
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 60
IP  - 4
DP  - 2005 Apr
TI  - IgE antibodies to omega-5 gliadin in children with wheat-induced anaphylaxis.
PG  - 506-9
AB  - BACKGROUND: Wheat can cause severe immunoglobulin E (IgE)-mediated systemic 
      reactions including anaphylaxis but knowledge on relevant wheat allergens at the 
      molecular level is scanty. METHODS: Seven children (aged from 6 months to 13 
      years) experiencing from 2 to 10 anaphylactic reactions in a year after eating 
      food-containing wheat were examined. Purified omega-5 gliadin was used as an 
      allergen in IgE enzyme-linked immunosorbent assay (ELISA) and in skin prick 
      testing (SPT). Wheat CAP radioallergosorbent test (RAST) and SPT were also 
      examined. RESULTS: All seven anaphylactic children, but none of 15 control 
      subjects had IgE antibodies to omega-5 gliadin in ELISA. Five of the six tested 
      anaphylactic children showed positive SPT to omega-5 and crude gliadin, and all 
      seven had positive wheat CAP RAST and SPT. One child was challenged with wheat, 
      which caused anaphylaxis. After adherence to a wheat-free diet four children 
      remained symptomless and three experienced one to two anaphylactic reactions. 
      CONCLUSION: The present results show that wheat omega-5 gliadin is a major 
      sensitizing allergen in children with wheat-induced anaphylaxis. They also 
      suggest that omega-5 gliadin IgE ELISA could be used as a diagnostic test for 
      this severe allergy.
FAU - Daengsuwan, T
AU  - Daengsuwan T
AD  - Department of Pediatrics, Faculty of Medicine, Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Palosuo, K
AU  - Palosuo K
FAU - Phankingthongkum, S
AU  - Phankingthongkum S
FAU - Visitsunthorn, N
AU  - Visitsunthorn N
FAU - Jirapongsananuruk, O
AU  - Jirapongsananuruk O
FAU - Alenius, H
AU  - Alenius H
FAU - Vichyanond, P
AU  - Vichyanond P
FAU - Reunala, T
AU  - Reunala T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (omega-5 gliadin, wheat)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-90-3 (Gliadin)
SB  - IM
MH  - Adolescent
MH  - Allergens/*immunology
MH  - Anaphylaxis/*blood/etiology
MH  - Antigens, Plant
MH  - Child
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gliadin/*immunology
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Infant
MH  - Male
MH  - Radioallergosorbent Test
MH  - Skin Tests
MH  - Wheat Hypersensitivity/*complications/*immunology
EDAT- 2005/02/25 09:00
MHDA- 2005/06/21 09:00
CRDT- 2005/02/25 09:00
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/06/21 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - ALL656 [pii]
AID - 10.1111/j.1398-9995.2004.00656.x [doi]
PST - ppublish
SO  - Allergy. 2005 Apr;60(4):506-9. doi: 10.1111/j.1398-9995.2004.00656.x.