PMID- 15727836
OWN - NLM
STAT- MEDLINE
DCOM- 20050419
LR  - 20061115
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 244
IP  - 1
DP  - 2005 Mar 1
TI  - Fast protocols for the 5S rDNA and ITS-2 based identification of Oenococcus oeni.
PG  - 165-71
AB  - To identify specific marker sequences for the rapid identification of Oenococcus 
      oeni, we sequenced the 23S-5S internal transcribed spacer (ITS-2) region and the 
      5S rDNA of five different O. oeni strains and three phylogenetically related 
      lactic acid bacteria (LAB). Comparative analysis revealed 100% identity among the 
      ITS-2 region of the O. oeni strains and remarkable differences in length and 
      sequence compared to related LAB. These results enabled us to develop a primer 
      set for a rapid PCR-identification of O. oeni within three hours. Moreover, the 
      comparison of the 5S rDNA sequences and the highly conserved secondary structure 
      provided the template for the design of three fluorescence-labeled specific 
      oligonucleotides for fluorescence in situ hybridization (FISH). These probes are 
      partial complementary to each other. This feature promotes the accessibility to 
      the target sequence within the ribosome and enhances the fluorescence signal. For 
      the rapid identification of Oenococci both the 5S rRNA gene and the ITS-2 region 
      are useful targets.
FAU - Hirschhauser, Steffen
AU  - Hirschhauser S
AD  - Institute for Microbiology and Wine Research, Johannes Gutenberg University of 
      Mainz, D-55128 Mainz, Germany.
FAU - Frohlich, Jurgen
AU  - Frohlich J
FAU - Gneipel, Armin
AU  - Gneipel A
FAU - Schonig, Inge
AU  - Schonig I
FAU - Konig, Helmut
AU  - Konig H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Base Sequence
MH  - DNA, Bacterial/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - DNA, Ribosomal Spacer/genetics
MH  - Genes, Bacterial
MH  - Gram-Positive Cocci/classification/*genetics/*isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - Leuconostoc/classification/*genetics/*isolation & purification
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Bacterial/chemistry/genetics
MH  - RNA, Ribosomal, 5S/chemistry/genetics
MH  - Sequence Homology, Nucleic Acid
EDAT- 2005/02/25 09:00
MHDA- 2005/04/20 09:00
CRDT- 2005/02/25 09:00
PHST- 2004/08/02 00:00 [received]
PHST- 2004/11/30 00:00 [revised]
PHST- 2005/01/20 00:00 [accepted]
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/04/20 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - S0378-1097(05)00059-5 [pii]
AID - 10.1016/j.femsle.2005.01.033 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2005 Mar 1;244(1):165-71. doi: 10.1016/j.femsle.2005.01.033.