PMID- 15729409
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 48
IP  - 1
DP  - 2005 Feb
TI  - Genomic characterization and physical mapping of two fucosyltransferase genes in 
      Medicago truncatula.
PG  - 168-76
AB  - Fucosyltransferases catalyse fucose transfer onto oligosaccharides. Two 
      fucosylated structures have been identified in plants: the alpha1,4-fucosylated 
      Lewis-a epitope and the alpha1,3-fucosylated core. Here we report the cloning, 
      genomic characterization, and physical mapping of two genes encoding proteins 
      similar to alpha1,4-fucosyltransferase (EC 2.4.1.65, MtFUT1) and 
      alpha1,3-fucosyltransferase (EC 2.4.1.214, MtFUT2) in Medicago truncatula. 
      Analysis of the genomic organization of the fucosyltransferase genes in M. 
      truncatula, revealed the presence of two genomic variants of the MtFUT1 gene 
      coding sequence, one containing a single intron and the other intronless, whereas 
      in MtFUT2, the gene coding region is interrupted by four introns. Using for the 
      first time fluorescence in situ hybridization (FISH) to physically map 
      fucosyltransferase genes in plants, this study reveals a high genomic dispersion 
      of these genes in Medicago. The MtFUT1 genes are mapped on chromosomes 4, 7, and 
      8, colocalizing on three of the five MtFUT2 loci. Chromosomes 1 and 5 carry the 
      additional MtFUT2 loci. Moreover, the intensity of the FISH signals reveals 
      marked differences in the number of gene copies per locus for both genes. 
      Simultaneous mapping of rRNA genes on chromosome 5 shows that several MTFUT2 gene 
      loci are inserted within the rDNA array. Insertions of coding DNA sequences into 
      the rDNA repeats were never reported to date.
FAU - Castilho, Alexandra
AU  - Castilho A
AD  - Centro de Botanica Aplicada a Agricultura, Instituto Superior de Agronomia, 
      Lisboa, Portugal. castilho@isa.utl.pt
FAU - Cunha, Margarida
AU  - Cunha M
FAU - Afonso, Ana Rita
AU  - Afonso AR
FAU - Morais-Cecilio, Leonor
AU  - Morais-Cecilio L
FAU - Fevereiro, Pedro S
AU  - Fevereiro PS
FAU - Viegas, Wanda
AU  - Viegas W
LA  - eng
SI  - GENBANK/AY553062
SI  - GENBANK/AY557602
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - EC 2.4.1.- (Fucosyltransferases)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Fucosyltransferases/*genetics
MH  - *Genes, Plant
MH  - *Genome, Plant
MH  - Medicago/enzymology/*genetics
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping
EDAT- 2005/02/25 09:00
MHDA- 2005/08/03 09:00
CRDT- 2005/02/25 09:00
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - g04-094 [pii]
AID - 10.1139/g04-094 [doi]
PST - ppublish
SO  - Genome. 2005 Feb;48(1):168-76. doi: 10.1139/g04-094.