PMID- 15729695
OWN - NLM
STAT- MEDLINE
DCOM- 20050629
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 115
IP  - 5
DP  - 2005 Jul 10
TI  - Real-time RT-PCR detection of CK19, CK7 and MUC1 mRNA for diagnosis of lymph node 
      micrometastases in non small cell lung carcinoma.
PG  - 777-82
AB  - Metastatic lymph nodes (LNs) are the major prognostic factor in resected non 
      small cell lung carcinoma (NSCLC). However, almost 50% of pN0 patients relapse, 
      suggesting metastatic cells undetected by current staging procedures. A 
      combination of markers [cytokeratins 19 and 7 (CK19, CK7) and mucin type 1 (MUC1) 
      mRNAs] was therefore evaluated by real-time RT-PCR in order to detect occult 
      cancer cells. Forty-three NSCLC tumor samples, 4 micrometastatic, 6 metastatic 
      and 84 histologically negative mediastinal LNs from 19 patients with NSCLC were 
      evaluated as well as blood mononuclear cells from 29 healthy volunteers and 17 
      benign LNs. When tested on cell lines, RT-PCR was particularly efficient for 
      evaluation of CK19, CK7 and MUC1 mRNA expression. All tumor samples were positive 
      for at least 1 marker and 74% of samples were positive for all 3 markers. CK7 and 
      CK19 mRNA were not detected in benign LN and blood cells from healthy donors in 
      contrast with MUC1 mRNA. Only CK7 and CK19 mRNA were therefore used for 
      evaluation of mediastinal LNs: the 6 histologically metastatic and the 4 
      micrometastatic LNs were positive for at least one marker. Among the 84 
      histologically negative LNs, 6 (7%) were positive for at least one marker, 
      potentially changing the stage of 2 out of 19 patients. In conclusion, in our 
      feasibility study, parallel molecular detection of CK19 and CK7 mRNA can be 
      considered a specific diagnostic tool for the assessment of microscopic lymphatic 
      spread. Its prognostic impact remains to be evaluated in a prospective study.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Saintigny, Pierre
AU  - Saintigny P
AD  - Histologie-Biologie Tumorale, UPRES EA 3499, Universite Paris 6, Hopital Tenon, 
      Paris, France. pierre.saintigny@avc.ap-hop-paris.fr
FAU - Coulon, Severine
AU  - Coulon S
FAU - Kambouchner, Marianne
AU  - Kambouchner M
FAU - Ricci, Sylvie
AU  - Ricci S
FAU - Martinot, Emmanuel
AU  - Martinot E
FAU - Danel, Claire
AU  - Danel C
FAU - Breau, Jean-Luc
AU  - Breau JL
FAU - Bernaudin, Jean-Francois
AU  - Bernaudin JF
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (KRT7 protein, human)
RN  - 0 (Keratin-7)
RN  - 0 (Mucin-1)
RN  - 0 (RNA, Messenger)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Non-Small-Cell Lung/diagnosis/genetics/*pathology
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratin-7
MH  - Keratins/analysis/*biosynthesis/*genetics
MH  - Lung Neoplasms/diagnosis/genetics/*pathology
MH  - Lymphatic Metastasis/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Mucin-1/analysis/*biosynthesis/*genetics
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
EDAT- 2005/02/25 09:00
MHDA- 2005/06/30 09:00
CRDT- 2005/02/25 09:00
PHST- 2005/02/25 09:00 [pubmed]
PHST- 2005/06/30 09:00 [medline]
PHST- 2005/02/25 09:00 [entrez]
AID - 10.1002/ijc.20942 [doi]
PST - ppublish
SO  - Int J Cancer. 2005 Jul 10;115(5):777-82. doi: 10.1002/ijc.20942.