PMID- 15730393
OWN - NLM
STAT- MEDLINE
DCOM- 20050414
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 139
IP  - 3
DP  - 2005 Mar
TI  - Engagement of human monocyte-derived dendritic cells into interleukin (IL)-12 
      producers by IL-1beta + interferon (IFN)-gamma.
PG  - 476-82
AB  - Dendritic cells (DCs) are potent antigen-presenting cells and can induce tumour- 
      or pathogen-specific T cell responses. For adoptive immunotherapy purposes, 
      immature DCs can be generated from adherent monocytes using granulocyte 
      macrophage colony stimulating factor (GM-CSF) and interleukin (IL)-4, and further 
      maturation is usually achieved by incubation with tumour necrosis factor 
      (TNF)-alpha. However, TNF-alpha-stimulated DCs produce low levels of IL-12. In 
      this study, we compared the effects of TNF-alpha, interferon (IFN)-gamma, 
      IL-1beta or IFN-gamma + IL-1beta on the phenotypic and functional maturation of 
      DCs. Our results show that IFN-gamma, but not IL-1beta, augmented the surface 
      expression of CD80, CD83 and CD86 molecules without inducing IL-12 production 
      from DCs. However, IL-1beta, but not IFN-gamma, induced IL-12 p40 production by 
      DCs without enhancing phenotypic maturation. When combined, IFN-gamma + IL-1beta 
      treatment profoundly up-regulated the expression of CD80, CD83, CD86 and major 
      histocompatibility complex (MHC) class II antigens. Furthermore, IFN-gamma + 
      IL-1beta-treated DCs produced larger amounts of IL-12 and induced stronger T cell 
      proliferation and IFN-gamma secretion in primary allogeneic mixed lymphocyte 
      reaction (MLR) than did TNF-alpha-treated DCs. Our results show that IFN-gamma + 
      IL-1beta induced human monocyte-derived DCs to differentiate into Th1-prone 
      mature DCs.
FAU - Nakahara, T
AU  - Nakahara T
AD  - Department of Dermatology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan. nakahara@dermatol.med.kyushu-u.ac.jp
FAU - Urabe, K
AU  - Urabe K
FAU - Fukagawa, S
AU  - Fukagawa S
FAU - Uchi, H
AU  - Uchi H
FAU - Inaba, K
AU  - Inaba K
FAU - Furue, M
AU  - Furue M
FAU - Moroi, Y
AU  - Moroi Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/*immunology/transplantation
MH  - Flow Cytometry
MH  - Humans
MH  - Immunotherapy, Adoptive/*methods
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-1/*pharmacology
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-12/*biosynthesis
MH  - Lymphocyte Culture Test, Mixed
MH  - Neoplasms/immunology/*therapy
MH  - Transplantation, Homologous
MH  - Tumor Necrosis Factor-alpha/biosynthesis
PMC - PMC1809303
EDAT- 2005/02/26 09:00
MHDA- 2005/04/15 09:00
PMCR- 2006/03/01
CRDT- 2005/02/26 09:00
PHST- 2005/02/26 09:00 [pubmed]
PHST- 2005/04/15 09:00 [medline]
PHST- 2005/02/26 09:00 [entrez]
PHST- 2006/03/01 00:00 [pmc-release]
AID - CEI2709 [pii]
AID - 10.1111/j.1365-2249.2004.02709.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2005 Mar;139(3):476-82. doi: 10.1111/j.1365-2249.2004.02709.x.