PMID- 15734755
OWN - NLM
STAT- MEDLINE
DCOM- 20050929
LR  - 20191210
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 20
IP  - 6
DP  - 2005 Jun
TI  - Secretion of cytokines and chemokines by polarized human epithelial cells from 
      the female reproductive tract.
PG  - 1439-46
AB  - BACKGROUND: Pro-inflammatory chemokines that attract and cytokines that activate 
      immune cells contribute to normal physiological homeostasis in the female 
      reproductive tract, and are needed to deal effectively with potential pathogenic 
      microbes. Mucosal epithelial cells are capable of producing these factors that 
      communicate with cells of the innate and adaptive immune systems. METHODS: 
      Epithelial cells from Fallopian tube, endometrium and endocervix were isolated 
      and grown to high transepithelial resistance in cell inserts from seven patients 
      who had hysterectomies. Interleukin (IL)-8, IL-6, granulocyte colony-stimulating 
      factor (G-CSF), monocyte chemoattractant protein-1 (MCP-1), 
      granulocyte-macrophage colony-stimulating factor (GM-CSF), tumour necrosis 
      factor-alpha (TNF-alpha) and macrophage inflammatory peptide-1beta (MIP-1beta) 
      were assessed by Luminex bead analysis or enzyme-linked immunosorbent assay 
      (ELISA) in epithelial cell conditioned media from the apical and basolateral 
      compartments. RESULTS: With the exception of MCP-1, the seven 
      chemokines/cytokines constitutively produced by the polarized epithelial cells 
      were preferentially secreted apically. A concentration pattern was found in all 
      cases, with IL-8 and IL-6 produced in the greatest quantity. CONCLUSIONS: The 
      concentrations of IL-8, IL-6, G-CSF and MCP-1 are similar to the levels found in 
      reproductive tract fluids of patients with infection. The constitutive secretion 
      and compartmentalization of large quantities of bioactive chemokines and 
      cytokines provide additional evidence for the role of epithelial cells as 
      gatekeepers of innate immune protection in the female reproductive tract.
FAU - Fahey, J V
AU  - Fahey JV
AD  - Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USA. 
      John.V.Fahey@Dartmouth.edu
FAU - Schaefer, T M
AU  - Schaefer TM
FAU - Channon, J Y
AU  - Channon JY
FAU - Wira, C R
AU  - Wira CR
LA  - eng
GR  - AI51877/AI/NIAID NIH HHS/United States
GR  - CA23108/CA/NCI NIH HHS/United States
GR  - T32 AI07363-12/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050225
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cell Polarity
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/*metabolism
MH  - Cytokines/*metabolism
MH  - Epithelial Cells/*metabolism/physiology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Infections/pathology
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Mucous Membrane/cytology
MH  - Uterus/*cytology/metabolism
EDAT- 2005/03/01 09:00
MHDA- 2005/09/30 09:00
CRDT- 2005/03/01 09:00
PHST- 2005/03/01 09:00 [pubmed]
PHST- 2005/09/30 09:00 [medline]
PHST- 2005/03/01 09:00 [entrez]
AID - deh806 [pii]
AID - 10.1093/humrep/deh806 [doi]
PST - ppublish
SO  - Hum Reprod. 2005 Jun;20(6):1439-46. doi: 10.1093/humrep/deh806. Epub 2005 Feb 25.