PMID- 15735559
OWN - NLM
STAT- MEDLINE
DCOM- 20050711
LR  - 20061115
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 11
IP  - 3
DP  - 2005 Mar
TI  - Co-localization of human herpes virus 6 and tissue plasminogen activator in 
      multiple sclerosis brain tissue.
PG  - BR84-7
AB  - BACKGROUND: Multiple sclerosis (MS) is a chronic inflammatory disease of the 
      central nervous system of unknown etiology. Several viruses have been suggested 
      as playing a role in the pathogenesis of MS. The aim of this study was to 
      investigate the interrelationship of human herpesvirus 6 (HHV-6) and plasminogen 
      activation at the cellular level in MS plaques. MATERIAL/METHODS: Brain tissue 
      specimens obtained from autopsies of 15 patients with MS and 10 controls were 
      studied immunohistochemically for HHV-6 and cytomegalovirus (CMV) antigen and 
      tissue plasminogen activator (tPA) protein. The presence of Ebstein-Barr virus 
      (EBV) EBER RNA was studied using RNA in situ hybridization. RESULTS: HHV-6 
      antigen was identified in the cells of 67% (10/15) of MS brain sections and 30% 
      (3/10) of the control sections. All samples were negative for CMV antigen and all 
      samples with intact RNA were negative for EBV EBER RNA as demonstrated by in situ 
      hybridization. tPA expression was found to be increased in MS plaques compared 
      with the control samples. Interestingly, in 5 MS samples both HHV-6 antigen and 
      tPA stained clearly, compared with none in the controls, but HHV-6 and tPA only 
      occasionally co-localized in the same cells. CONCLUSIONS: At the cellular level, 
      HHV-6 and plasminogen activation seem to co-localize in MS.
FAU - Virtanen, Jussi Oskari
AU  - Virtanen JO
AD  - Haartman Institute, Department of Virology, University of Helsinki, Finland.
FAU - Zabriskie, John B
AU  - Zabriskie JB
FAU - Siren, Vappu
AU  - Siren V
FAU - Friedman, Jacqueline E
AU  - Friedman JE
FAU - Lyons, Michael J
AU  - Lyons MJ
FAU - Edgar, Mark
AU  - Edgar M
FAU - Vaheri, Antti
AU  - Vaheri A
FAU - Koskiniemi, Marjaleena
AU  - Koskiniemi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Antigens, Viral)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - Brain/*virology
MH  - Case-Control Studies
MH  - Herpesvirus 6, Human/*genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Middle Aged
MH  - Multiple Sclerosis/*virology
MH  - Tissue Plasminogen Activator/*metabolism
EDAT- 2005/03/01 09:00
MHDA- 2005/07/12 09:00
CRDT- 2005/03/01 09:00
PHST- 2004/06/03 00:00 [received]
PHST- 2004/07/02 00:00 [accepted]
PHST- 2005/03/01 09:00 [pubmed]
PHST- 2005/07/12 09:00 [medline]
PHST- 2005/03/01 09:00 [entrez]
AID - 5648 [pii]
PST - ppublish
SO  - Med Sci Monit. 2005 Mar;11(3):BR84-7.