PMID- 15735940
OWN - NLM
STAT- MEDLINE
DCOM- 20050912
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 49
IP  - 2
DP  - 2005 Feb
TI  - Molecular, biochemical, and physiological approaches for understanding the 
      ecology of denitrification.
PG  - 198-208
AB  - One of the major challenges in microbial ecology for the future is to establish 
      links between structural and functional biodiversity. This is particularly 
      difficult when one is interested in a phylogenetically diversified function such 
      as denitrification. The data banks are very rich in functional gene sequences 
      (nirS in this study), but most of them were obtained from not yet cultivated 
      bacteria, and thus must be supplemented by sequences of organisms from the 
      environment for which we could associate a taxonomic position and physiological 
      characteristics. Combined analysis including molecular (16S-rRNA or nirS genes), 
      physiological, and biochemical approaches was carried out on a bacterial set of 
      89 strains isolated from marine sediment. The denaturing gradient gel 
      electrophoresis (DGGE) technique was successfully applied on unclamped polymerase 
      chain reaction (PCR) products of nirS genes to compare the picture of the 
      biodiversity obtained with 16S rRNA and nirS genes. The diversity of nirS genes 
      and denitrifier characteristics were found within several of the 16S rDNA 
      phylotypes. In contrast, the nirS phylotypes were no diverse both with respect to 
      16S rDNA and to physiology and biochemistry of denitrification. Sequences of the 
      nirS PCR products were very close to marine environmental clones and were 
      analyzed within the same phylogenetic tree.
FAU - Goregues, C M
AU  - Goregues CM
AD  - Laboratoire de Microbiologie, de Geochimie et d'Ecologie Marines, CNRS-UMR 6117, 
      Centre d'Oceanologie de Marseille, Campus de Luminy, Case 90l, 13288, Marseille 
      cedex 9, France.
FAU - Michotey, V D
AU  - Michotey VD
FAU - Bonin, P C
AU  - Bonin PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050224
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria/*genetics/*metabolism
MH  - *Biodiversity
MH  - Ecosystem
MH  - Genes, Bacterial
MH  - Genetic Variation
MH  - Nitrogen/*metabolism
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2005/03/01 09:00
MHDA- 2005/09/13 09:00
CRDT- 2005/03/01 09:00
PHST- 2003/11/28 00:00 [received]
PHST- 2004/02/10 00:00 [accepted]
PHST- 2005/03/01 09:00 [pubmed]
PHST- 2005/09/13 09:00 [medline]
PHST- 2005/03/01 09:00 [entrez]
AID - 10.1007/s00248-004-0256-7 [doi]
PST - ppublish
SO  - Microb Ecol. 2005 Feb;49(2):198-208. doi: 10.1007/s00248-004-0256-7. Epub 2005 
      Feb 24.