PMID- 15737545
OWN - NLM
STAT- MEDLINE
DCOM- 20050907
LR  - 20131121
IS  - 0927-7765 (Print)
IS  - 0927-7765 (Linking)
VI  - 41
IP  - 2-3
DP  - 2005 Mar 25
TI  - Surfactant encapsulated DNA: structure characterization and interaction with dye 
      molecules in organic media.
PG  - 181-7
AB  - The recognition of electrostatically-bound DNA-didodecyldimethylammonium 
      (DNA-DDDA) complex by three dye molecules, acridine orange (AO), ethidium bromide 
      (EB) and 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin 
      tetra(p-toluenesulfonate) (TMPyP) in organic media was investigated through 1H 
      NMR, UV-vis, and circular dichroism (CD) spectroscopies. When the organic solvent 
      in which DNA-DDDA complex dissolves is changed from ethanol to chloroform, the 
      adsorbed AO undergoes a reversible transformation from a monomer to a highly 
      aggregated state at the interface between DNA and DDDA. EB also adsorbs at the 
      interface between DNA and DDDA when EB interacts with the DNA-DDDA complex in 
      organic media, but its existing state is independent of the used solvents. The 
      third dye, TMPyP cation can intercalate into the G-C region while its anionic 
      p-tosylate counterion remains unbound when it mixes with DNA complex in organic 
      media. The complexes of DDDA with previously recognized DNA by the three dye 
      molecules (DNA-dye), respectively, are also investigated. AO seems having changed 
      its location from the grooves of DNA to the interface between DNA and DDDA after 
      DNA-AO complex was electrostatically encapsulated with DDDA. The aggregation 
      behavior of AO also shows a dependence on the polarity of the organic solvent. EB 
      molecules are believed to intercalate into the base pairs of DNA in aqueous 
      solution. The intercalation mode is still maintained after the encapsulation for 
      DNA-EB in organic solvents, which is different from the situation between 
      DNA-DDDA complex and EB. But in both cases, the existing states of EB are 
      independent of the polarity of the organic solvents. Finally, TMPyP in the 
      complex of DNA-TMPyP and DDDA is also judged to intercalate into the G-C region 
      of DNA while its anionic p-tosylate counterion remains separated from DNA 
      complex, which is similar to its interaction with DNA-DDDA complex in organic 
      media. These data also strongly suggest that the intercalation state of TMPyP is 
      more stable than its adsorption state in grooves when it is recognized with DNA. 
      The present results are significant for the designs of both laser dye and 
      conductive materials.
FAU - Hou, Xueliang
AU  - Hou X
AD  - Key Laboratory for Supramolecular Structure and Materials of Ministry of 
      Education, Jilin University, No. 10 Qianwei Road, Changchun 130012, Jilin, PR 
      China.
FAU - Xu, Miao
AU  - Xu M
FAU - Wu, Lixin
AU  - Wu L
FAU - Shen, Jiacong
AU  - Shen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050119
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Coloring Agents)
RN  - 0 (Culture Media)
RN  - 0 (Organic Chemicals)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Solvents)
RN  - 0 (Surface-Active Agents)
RN  - 13146-86-6 (didodecyldimethylammonium)
RN  - 3K9958V90M (Ethanol)
RN  - 7V31YC746X (Chloroform)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Chloroform
MH  - Coloring Agents
MH  - Culture Media
MH  - DNA/chemistry/*ultrastructure
MH  - Ethanol
MH  - Fishes
MH  - Male
MH  - Nucleic Acid Conformation
MH  - Organic Chemicals
MH  - *Quaternary Ammonium Compounds
MH  - Solvents
MH  - Spectrophotometry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Static Electricity
MH  - Surface-Active Agents
MH  - Testis
EDAT- 2005/03/02 09:00
MHDA- 2005/09/08 09:00
CRDT- 2005/03/02 09:00
PHST- 2004/06/14 00:00 [received]
PHST- 2004/11/20 00:00 [revised]
PHST- 2004/12/17 00:00 [accepted]
PHST- 2005/03/02 09:00 [pubmed]
PHST- 2005/09/08 09:00 [medline]
PHST- 2005/03/02 09:00 [entrez]
AID - S0927-7765(04)00388-1 [pii]
AID - 10.1016/j.colsurfb.2004.12.005 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2005 Mar 25;41(2-3):181-7. doi: 
      10.1016/j.colsurfb.2004.12.005. Epub 2005 Jan 19.