PMID- 15739027
OWN - NLM
STAT- MEDLINE
DCOM- 20050603
LR  - 20201226
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 52
IP  - 1
DP  - 2005
TI  - No evidence of HTLV-I infection in patients with mycosis fungoides and Sezary 
      syndrome.
PG  - 52-5
AB  - The involvement of human T-cell lymphotropic virus type I (HTLV-I) in the 
      etiology of cutaneous T-cell lymphomas (CTCL) is still controversial. The aim of 
      the study was to evaluate the role of HTLV-I in the pathogenesis of mycosis 
      fungoides (MF) and Sezary syndrome (SS) in Polish patients. The studied group 
      consisted of 42 patients with MF, 5 with SS and 25 with chronic dermatitis. DNA 
      was extracted from snap-frozen and paraffin-embedded skin biopsies and from 
      peripheral blood. Polymerase chain reaction (PCR or nested PCR) was carried out 
      for amplification of different regions of HTLV-I genome. Primer sets flanking pX, 
      p 19, U5, tax and pol genes were used in the investigation. The presence of 
      HTLV-I antibody was examined in 46 sera samples with the use of anti-HTLV-I/II 
      EIA test. HTLV-I antibodies were not detected in any collected sera samples. PCR 
      with two primer sets homologous to the pX region of HTLV-I showed negative 
      results in all samples investigated. To confirm these results two other primer 
      pairs specific for U5 and gag regions were designed. With these primer pairs no 
      PCR product, except that in positive control, was observed. For more sensitive 
      amplification a nested-PCR with pol and tax specific primers was performed. 
      HTLV-I probably does not play an important role in the pathogenesis of MF in 
      Polish patients.
FAU - Pawlaczyk, M
AU  - Pawlaczyk M
AD  - Department of Biology and Environmental Studies, University of Medical Sciences, 
      Poznan, Poland. magdula2811@poczta.onet.pl
FAU - Filas, V
AU  - Filas V
FAU - Sobieska, M
AU  - Sobieska M
FAU - Gozdzicka-Jozefiak, A
AU  - Gozdzicka-Jozefiak A
FAU - Wiktorowicz, K
AU  - Wiktorowicz K
FAU - Breborowicz, J
AU  - Breborowicz J
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Antibodies, Viral/analysis
MH  - DNA, Viral/analysis
MH  - HTLV-I Infections/*complications
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - Mycosis Fungoides/*physiopathology/*virology
MH  - Polymerase Chain Reaction
MH  - Sezary Syndrome/*physiopathology/*virology
EDAT- 2005/03/02 09:00
MHDA- 2005/06/04 09:00
CRDT- 2005/03/02 09:00
PHST- 2005/03/02 09:00 [pubmed]
PHST- 2005/06/04 09:00 [medline]
PHST- 2005/03/02 09:00 [entrez]
PST - ppublish
SO  - Neoplasma. 2005;52(1):52-5.