PMID- 15739032
OWN - NLM
STAT- MEDLINE
DCOM- 20050603
LR  - 20131121
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 52
IP  - 1
DP  - 2005
TI  - Chromosome damage induced by benzene after the use of conventional and FISH 
      chromosome painting.
PG  - 79-84
AB  - The cytogenetic effects of in vitro exposure to benzene were investigated in 
      cultured bovine peripheral lymphocytes. Stable and unstable chromosome 
      aberrations (CA) and sister chromatid exchanges (SCE) were examined. Cultures of 
      lymphocytes from two healthy donors were treated with benzene at concentrations 
      of 5, 10, 50, 100, 500 and 1000 micromol.l-1 for the last 24 hours and for 2 
      hours of cultivation, both without and with metabolic activation (S9). In the CA 
      assays with conventional Giemsa staining for 24 hours without metabolic 
      activation, no dose-dependence in the induced chromosome aberration was achieved. 
      A significant elevation in the induction of CA was found only after the 
      application of benzene at a dose of 100 micromol.l-1 (p<0.01). Similar results 
      were observed using fluorescence in situ hybridization (FISH) method for the 
      detection both of non-stable and persistent chromatid-type aberrations and in the 
      SCE assay for 24 hours without S9. All the lower and higher concentrations tested 
      have no positive influence on CA and SCE induction. In experiments with S9 a 
      significant increase in CAs, and a dose-dependence was achieved after exposure to 
      benzene for 2 hours at concentrations ranging from 50 to 500 micromol.l-1 (p<0.05 
      and p<0.01, respectively).
FAU - Sivikova, K
AU  - Sivikova K
AD  - Institute of Genetics, University of Veterinary Medicine, 04181 Kosice, Slovak 
      Republic. sivikova@uvm.sk
FAU - Holeckova, B
AU  - Holeckova B
FAU - Dianovsky, J
AU  - Dianovsky J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - J64922108F (Benzene)
SB  - IM
MH  - Animals
MH  - Benzene/*toxicity
MH  - Cattle
MH  - Cell Culture Techniques
MH  - Chromosome Aberrations/*chemically induced
MH  - Chromosome Painting
MH  - DNA Damage/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - Sister Chromatid Exchange
EDAT- 2005/03/02 09:00
MHDA- 2005/06/04 09:00
CRDT- 2005/03/02 09:00
PHST- 2005/03/02 09:00 [pubmed]
PHST- 2005/06/04 09:00 [medline]
PHST- 2005/03/02 09:00 [entrez]
PST - ppublish
SO  - Neoplasma. 2005;52(1):79-84.