PMID- 15741765
OWN - NLM
STAT- MEDLINE
DCOM- 20050429
LR  - 20181201
IS  - 1010-4283 (Print)
IS  - 1010-4283 (Linking)
VI  - 26
IP  - 1
DP  - 2005 Jan-Feb
TI  - Characterization of HER1 (c-erbB1) status in locally advanced breast cancer using 
      fluorescence in situ hybridization and immunohistochemistry.
PG  - 25-30
AB  - Epidermal growth factor receptor (EGFR) is a 170-kDa transmembrane glycoprotein 
      encoded by the HER1 protooncogene, located at 7p12. This receptor is related to 
      the pathogenesis of breast cancer. The aim of this study was to analyze the 
      status of HER1 using fluorescence in situ hybridization (FISH) and 
      immunohistochemistry in a series of 48 patients with locally advanced breast 
      cancer (LABC). Before neoadjuvant chemotherapy, core biopsies were taken from 
      patients with LABC and were processed into paraffin blocks. Biopsies were then 
      studied using FISH with a HER1 probe (Vysis, Downers Grove, Ill., USA). They were 
      also analyzed immunohistochemically using two different EGFR antibodies from 
      DakoCytomation (Denmark, A/S) and from Zymed (San Francisco, Calif., USA). HER1 
      amplifications were not found, although 31% of the cases presented aneusomy of 
      chromosome 7. Only 2 cases presented EGFR expression. LABC presented a low level 
      of EGFR expression. HER1 amplification was not present in LABC, although the 
      polysomy of chromosome 7 was a common finding.
FAU - Corzo, Cristina
AU  - Corzo C
AD  - Laboratori de Citogenetica i Biologia molecular, Unitat de Recerca Traslacional 
      en Tumors Solids, Hospital del Mar, IMAS, Barcelona, Spain. E0062@imas.imim.es
FAU - Tusquets, Ignacio
AU  - Tusquets I
FAU - Salido, Marta
AU  - Salido M
FAU - Corominas, Josep Maria
AU  - Corominas JM
FAU - Bellet, Meritxell
AU  - Bellet M
FAU - Suarez, Marta
AU  - Suarez M
FAU - Baro, Teresa
AU  - Baro T
FAU - Fabregat, Xavier
AU  - Fabregat X
FAU - Serrano, Sergio
AU  - Serrano S
FAU - Sole, Francesc
AU  - Sole F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040228
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Breast Neoplasms/*genetics/metabolism
MH  - Chromosomes, Human, Pair 7
MH  - ErbB Receptors/analysis/*biosynthesis
MH  - Female
MH  - Gene Dosage
MH  - *Genes, erbB-1
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Spain
EDAT- 2005/03/03 09:00
MHDA- 2005/04/30 09:00
CRDT- 2005/03/03 09:00
PHST- 2004/07/13 00:00 [received]
PHST- 2004/10/19 00:00 [accepted]
PHST- 2005/03/03 09:00 [pubmed]
PHST- 2005/04/30 09:00 [medline]
PHST- 2005/03/03 09:00 [entrez]
AID - 84183 [pii]
AID - 10.1159/000084183 [doi]
PST - ppublish
SO  - Tumour Biol. 2005 Jan-Feb;26(1):25-30. doi: 10.1159/000084183. Epub 2004 Feb 28.