PMID- 15743507
OWN - NLM
STAT- MEDLINE
DCOM- 20060131
LR  - 20220408
IS  - 1465-542X (Electronic)
IS  - 1465-5411 (Print)
IS  - 1465-5411 (Linking)
VI  - 7
IP  - 2
DP  - 2005
TI  - Polysomy of chromosome 17 in breast cancer tumors showing an overexpression of 
      ERBB2: a study of 175 cases using fluorescence in situ hybridization and 
      immunohistochemistry.
PG  - R267-73
AB  - INTRODUCTION: One of the most common genetic aberrations associated with breast 
      cancer is the amplification and overexpression of the ERBB2 proto-oncogene 
      located at chromosome 17, bands q12-21. The amplification/overexpression occurs 
      in 25 to 30% of all breast cancers. In breast cancer, aneusomy of chromosome 17, 
      either monosomy or polysomy, is frequently observed by conventional cytogenetics 
      and fluorescence in situ hybridization (FISH). The aim of this study was to 
      discover whether or not numerical aberrations on chromosome 17 have a correlation 
      to the amplification or overexpression of the ERBB2 gene and to analyze their 
      clinical implications in subgroups showing 2+ or 3+ positive scores by 
      immunohistochemistry (IHC). METHODS: We used FISH on a series of 175 
      formalin-fixed paraffin-embedded breast carcinomas to detect ERBB2 amplification, 
      using a dual-probe system for the simultaneous enumeration of the ERBB2 gene and 
      the centromeric region of chromosome 17, as well as using IHC to detect 
      overexpression. We analyzed clinical and pathological variables in a subgroup of 
      patients with 2+ and 3+ IHC scores (147 patients), to describe any differences in 
      clinicopathological characteristics between polysomic and non-polysomic cases 
      with the use of the chi2 test. RESULTS: We found 13% of cases presenting 
      polysomy, and three cases presented monosomy 17 (2%). According to the status of 
      the ERBB2 gene, instances of polysomy 17 were more frequently observed in 
      non-amplified cases than in FISH-amplified cases, suggesting that the mechanism 
      for ERBB2 amplification is independent of polysomy 17. Polysomy 17 was detected 
      in patients with 2+ and 3+ IHC scores. We found that nodal involvement was more 
      frequent in polysomic than in non-polysomic cases (P = 0.046). CONCLUSIONS: The 
      determination of the copy number of chromosome 17 should be incorporated into the 
      assesment of ERBB2 status. It might also be helpful to differentiate a subgroup 
      of breast cancer patients with polysomy of chromosome 17 and overexpression of 
      ERBB2 protein that probably have genetic and clinical differences.
FAU - Salido, Marta
AU  - Salido M
AD  - Laboratori de Citogenetica i Biologia Molecular, Servei de Patologia, Hospital 
      del Mar, IMAS, Barcelona, Spain. MSALIDO@IMAS.IMIM.ES
FAU - Tusquets, Ignasi
AU  - Tusquets I
FAU - Corominas, Josep M
AU  - Corominas JM
FAU - Suarez, Marta
AU  - Suarez M
FAU - Espinet, Blanca
AU  - Espinet B
FAU - Corzo, Cristina
AU  - Corzo C
FAU - Bellet, Meritxell
AU  - Bellet M
FAU - Fabregat, Xavier
AU  - Fabregat X
FAU - Serrano, Sergi
AU  - Serrano S
FAU - Sole, Francesc
AU  - Sole F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050126
PL  - England
TA  - Breast Cancer Res
JT  - Breast cancer research : BCR
JID - 100927353
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 18
MH  - Female
MH  - Gene Amplification
MH  - Gene Dosage
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Prospective Studies
MH  - Proto-Oncogene Mas
MH  - Receptor, ErbB-2/*biosynthesis/genetics
MH  - Up-Regulation
PMC - PMC1064140
EDAT- 2005/03/04 09:00
MHDA- 2006/02/01 09:00
PMCR- 2005/01/26
CRDT- 2005/03/04 09:00
PHST- 2004/08/19 00:00 [received]
PHST- 2004/11/08 00:00 [revised]
PHST- 2005/01/05 00:00 [accepted]
PHST- 2005/03/04 09:00 [pubmed]
PHST- 2006/02/01 09:00 [medline]
PHST- 2005/03/04 09:00 [entrez]
PHST- 2005/01/26 00:00 [pmc-release]
AID - bcr996 [pii]
AID - 10.1186/bcr996 [doi]
PST - ppublish
SO  - Breast Cancer Res. 2005;7(2):R267-73. doi: 10.1186/bcr996. Epub 2005 Jan 26.