PMID- 15743749
OWN - NLM
STAT- MEDLINE
DCOM- 20050512
LR  - 20190415
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 123
IP  - 4
DP  - 2005 Apr
TI  - Multitarget FISH analysis in the diagnosis of lung cancer.
PG  - 516-23
AB  - The aim of the present study was to explore the diagnostic usefulness of the 
      multitarget fluorescence in situ hybridization (FISH) test, LAVysion (Vysis, 
      Downers Grove, IL), for the detection of lung cancer cells in cytologic 
      specimens. Specimens from bronchial washings, bronchial brushings, and 
      transbronchial fine-needle aspirates (TBNAs) from 100 patients with suspected 
      lung cancer and from a control group of 71 patients with nonneoplastic lung 
      disorders were analyzed. FISH positivity was defined as more than 5 cells with 
      gains of at least 2 chromosomes or gene loci. FISH significantly improved the 
      sensitivity of bronchial brushings from 49% to 73%. The specificities of FISH and 
      cytologic examination were 87% and 100%, respectively. In contrast, FISH provided 
      no additional diagnostic information in TBNAs and bronchial washings. There was 
      no increased prevalence of genetic changes in contralateral bronchial washings 
      from patients with lung cancer compared with the control group. The quantity of 
      previous smoking had no effect on the prevalence of chromosomal changes.
FAU - Bubendorf, Lukas
AU  - Bubendorf L
AD  - Institute for Pathology, University Hospital, Basel, Switzerland.
FAU - Muller, Phaedra
AU  - Muller P
FAU - Joos, Ladina
AU  - Joos L
FAU - Grilli, Bruno
AU  - Grilli B
FAU - Vogel, Sandrine
AU  - Vogel S
FAU - Herzog, Michelle
AU  - Herzog M
FAU - Barascud, Audrey
AU  - Barascud A
FAU - Feichter, Georg
AU  - Feichter G
FAU - Dalquen, Peter
AU  - Dalquen P
FAU - Tamm, Michael
AU  - Tamm M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Fine-Needle
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Smoking
EDAT- 2005/03/04 09:00
MHDA- 2005/05/13 09:00
CRDT- 2005/03/04 09:00
PHST- 2005/03/04 09:00 [pubmed]
PHST- 2005/05/13 09:00 [medline]
PHST- 2005/03/04 09:00 [entrez]
AID - F5ADJUWVD2WFL8J5 [pii]
AID - 10.1309/F5AD-JUWV-D2WF-L8J5 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2005 Apr;123(4):516-23. doi: 10.1309/F5AD-JUWV-D2WF-L8J5.