PMID- 15744759
OWN - NLM
STAT- MEDLINE
DCOM- 20050714
LR  - 20131121
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 25
IP  - 2
DP  - 2005 Mar-Apr
TI  - Novel approach for classifying chemicals according to skin sensitizing potency by 
      non-radioisotopic modification of the local lymph node assay.
PG  - 129-34
AB  - The murine local lymph node assay (LLNA) is currently recognized as a stand-alone 
      sensitization test for determining the sensitizing potential of chemicals, and it 
      has the advantage of yielding a quantitative endpoint that can be used to predict 
      the sensitization potency of chemicals. The EC3 has been proposed as a parameter 
      for classifying chemicals according to the sensitization potency. We previously 
      developed a non-radioisotopic endpoint for the LLNA based on 
      5-bromo-2'-deoxyuridine (BrdU) incorporation (non-RI LLNA), and we are proposing 
      a new procedure to predict the sensitization potency of chemicals based on 
      comparisons with known human contact allergens. Nine chemicals (i.e. 
      diphencyclopropenone, p-phenylenediamine, glutaraldehyde, cinnamicaldehyde, 
      citral, eugenol, isopropyl myristate, propyleneglycol and hexane) categorized as 
      human contact allergen classes 1-5 were tested by the non-RI LLNA with the 
      following reference allergens: 2,4-dinitrochlorobenzene (DNCB) as a class 1 human 
      contact allergen, isoeugenol as a class 2 human contact allergen and 
      alpha-hexylcinnamic aldehyde (HCA) as a class 3 human contact allergen. 
      Consequently, nine test chemicals were almost assigned to their correct allergen 
      class. The results suggested that the new procedure for non-RI LLNA can provide 
      correct sensitization potency data. Sensitization potency data are useful for 
      evaluating the sensitization risk to humans of exposure to new chemical products. 
      Accordingly, this approach would be an effective modification of LLNA with regard 
      to its experimental design. Moreover, this procedure can be applied also to the 
      standard LLNA with radioisotopes and to other modifications of the LLNA.
CI  - Copyright 2005 John Wiley & Sons, Ltd.
FAU - Takeyoshi, Masahiro
AU  - Takeyoshi M
AD  - Hita Laboratory, Chemicals Evaluation and Research Institute, 3-822 Ishii-machi, 
      Hita-shi, Oita 8770061, Japan. takeyoshi-masahiro@ceri.jp
FAU - Iida, Kenji
AU  - Iida K
FAU - Shiraishi, Keiji
AU  - Shiraishi K
FAU - Hoshuyama, Satsuki
AU  - Hoshuyama S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Allergens)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Allergens/*classification/*toxicity
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Dermatitis, Allergic Contact/etiology/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/drug effects/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Predictive Value of Tests
MH  - Reference Standards
MH  - Sensitivity and Specificity
MH  - Skin Tests/*methods
EDAT- 2005/03/04 09:00
MHDA- 2005/07/15 09:00
CRDT- 2005/03/04 09:00
PHST- 2005/03/04 09:00 [pubmed]
PHST- 2005/07/15 09:00 [medline]
PHST- 2005/03/04 09:00 [entrez]
AID - 10.1002/jat.1045 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2005 Mar-Apr;25(2):129-34. doi: 10.1002/jat.1045.