PMID- 15745225
OWN - NLM
STAT- MEDLINE
DCOM- 20050705
LR  - 20191210
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 69
IP  - 1
DP  - 2005 Jan
TI  - An evaluation of disinfectants for the sanitation of porcine reproductive and 
      respiratory syndrome virus-contaminated transport vehicles at cold temperatures.
PG  - 64-70
AB  - The objective of this study was to evaluate the efficacy of commercially 
      available disinfectants to sanitize porcine reproductive and respiratory syndrome 
      virus (PRRSV) contaminated trailer models in cold climates (-20 degrees C and 4 
      degrees C). Disinfectants evaluated included Synergize, Aseptol 2000, Biophene, 
      Sentramax, Virkon, Tek Trol, and DC&R. All products were applied to trailers via 
      fumigation at 4 degrees C. Following experimental contamination of model trailers 
      with PRRSV MN 30-100 (5 x 10(5) TCID50), models were tested for the presence or 
      absence of PRRSV-RNA by polymerase chain reaction (PCR) on swabs collected 0, 30, 
      and 60 min after treatment. Treatments included washing only, washing plus 
      disinfectant fumigation, washing plus fumigation, and washing plus overnight 
      drying. The PRRSV-RNA detected across trailers ranged from 0/12 replicates in 
      trailers treated with Synergize or allowed to dry for 8 h. These trailers were 
      also negative for the presence of infectious PRRSV, based on the lack of sentinel 
      pig infection (0/4 replicates). In contrast, the detection of PRRSV-positive 
      swabs by PCR ranged from 3/12 (Aseptol) to 10/12 (Biophene). Based on these 
      results, the efficacy of Synergize was evaluated at -20 degrees C. In an attempt 
      to reduce the impact of freezing on disinfectant activity, 30 mL of disinfectant 
      was added to a 3840 mL of a 40% methanol solution, a 10% propylene glycol (PG) 
      solution, or water alone. The PRRSV-contaminated trailers were treated with 1 of 
      3 disinfectant mixtures via fumigation, stored for 8 h at -20 degrees C, allowed 
      to thaw, and sampled as described. Trailers treated with 40% methanol or 10% PG 
      did not freeze and were negative for PRRSV-RNA and infectious virus following 
      thawing. In contrast, trailers treated with disinfectant and water were frozen 
      within 60 min at -20 degrees C, and decontamination was not successful.
FAU - Dee, Scott
AU  - Dee S
AD  - Swine Disease Eradication Center, 385 C Animal Science/Veterinary Medicine 
      Building, University of Minnesota College of Veterinary Medicine, 1988 Fitch 
      Avenue, St. Paul, Minnesota 55108, USA. deexx004@umn.edu
FAU - Deen, John
AU  - Deen J
FAU - Burns, Danny
AU  - Burns D
FAU - Douthit, George
AU  - Douthit G
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Disinfectants)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Biological Assay/veterinary
MH  - *Cold Temperature
MH  - Disease Reservoirs/veterinary
MH  - Disinfectants/*pharmacology
MH  - Disinfection/*methods
MH  - Polymerase Chain Reaction/methods/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control/transmission
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects/isolation & 
      purification
MH  - RNA, Viral/analysis
MH  - Swine
MH  - Time Factors
MH  - *Transportation
MH  - Treatment Outcome
PMC - PMC1142172
EDAT- 2005/03/05 09:00
MHDA- 2005/07/06 09:00
PMCR- 2005/04/01
CRDT- 2005/03/05 09:00
PHST- 2005/03/05 09:00 [pubmed]
PHST- 2005/07/06 09:00 [medline]
PHST- 2005/03/05 09:00 [entrez]
PHST- 2005/04/01 00:00 [pmc-release]
AID - pg64 [pii]
PST - ppublish
SO  - Can J Vet Res. 2005 Jan;69(1):64-70.