PMID- 15745956
OWN - NLM
STAT- MEDLINE
DCOM- 20060331
LR  - 20190912
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 25
IP  - 9
DP  - 2005 Mar 2
TI  - NMDA receptor-dependent regulation of axonal and dendritic branching.
PG  - 2304-11
AB  - In the rodent trigeminal principal nucleus (PrV), trigeminal afferent terminals 
      and postsynaptic cells form discrete modules ("barrelettes") that replicate the 
      patterned array of whiskers and sinus hairs on the snout. Barrelette neurons of 
      the PrV relay whisker-specific patterns to the contralateral thalamus and, 
      subsequently, to the primary somatosensory barrel cortex. Genetic impairment of 
      NMDA receptor (NMDAR) function blocks development of barrelettes in the PrV. 
      Underlying cellular and functional defects are not known. Here, we examined 
      morphological differentiation of whisker afferents, dendritic differentiation of 
      barrelette cells, and their electrophysiological properties in mice with genetic 
      perturbations of the essential subunit NR1 of NMDARs. We show that in NR1 gene 
      knock-down (KD) and knock-out mice, whisker afferents begin their embryonic 
      development normally but, over time, fail to segregate into patches, and instead 
      they develop exuberant terminal arbors spanning most of the PrV. Postnatal NR1KD 
      barrelette cells, with significantly reduced NMDA currents, retain their membrane 
      and synaptic properties but develop longer dendrites with no orientation 
      preference. These results indicate that NMDARs regulate growth of presynaptic 
      terminal arbors and postsynaptic dendritic branching, thereby leading to 
      consolidation of synapses and patterning of presynaptic and postsynaptic 
      elements.
FAU - Lee, Li-Jen
AU  - Lee LJ
AD  - Department of Cell Biology and Anatomy, Louisiana State University Health 
      Sciences Center, New Orleans, Louisiana 70112, USA.
FAU - Lo, Fu-Sun
AU  - Lo FS
FAU - Erzurumlu, Reha S
AU  - Erzurumlu RS
LA  - eng
GR  - R01 NS037070/NS/NINDS NIH HHS/United States
GR  - R01 NS039050/NS/NINDS NIH HHS/United States
GR  - NS37070/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (GABA Antagonists)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 62T278S1MX (FG 9041)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Axons/*physiology/ultrastructure
MH  - Bicuculline/pharmacology
MH  - Dendrites/*physiology/ultrastructure
MH  - Drug Interactions
MH  - Electron Transport Complex IV/metabolism
MH  - Embryo, Mammalian
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - GABA Antagonists/pharmacology
MH  - Membrane Potentials/physiology/radiation effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neurons/cytology/*physiology/ultrastructure
MH  - Patch-Clamp Techniques/methods
MH  - Quinoxalines/pharmacology
MH  - Receptors, N-Methyl-D-Aspartate/deficiency/*physiology
MH  - Silver Staining/methods
MH  - Trigeminal Nuclei/*cytology
MH  - Vibrissae/cytology/growth & development/innervation
PMC - PMC3556734
MID - NIHMS428531
EDAT- 2005/03/05 09:00
MHDA- 2006/04/01 09:00
PMCR- 2005/09/02
CRDT- 2005/03/05 09:00
PHST- 2005/03/05 09:00 [pubmed]
PHST- 2006/04/01 09:00 [medline]
PHST- 2005/03/05 09:00 [entrez]
PHST- 2005/09/02 00:00 [pmc-release]
AID - 25/9/2304 [pii]
AID - 10.1523/JNEUROSCI.4902-04.2005 [doi]
PST - ppublish
SO  - J Neurosci. 2005 Mar 2;25(9):2304-11. doi: 10.1523/JNEUROSCI.4902-04.2005.