PMID- 15748938
OWN - NLM
STAT- MEDLINE
DCOM- 20050527
LR  - 20071115
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 161
IP  - 1-2
DP  - 2005 Apr
TI  - Low immunogenicity of in vitro-expanded human neural cells despite high MHC 
      expression.
PG  - 1-11
AB  - The ability to expand human neural precursor cells in vitro offers new 
      possibilities for future cell therapies. However, concern over immunologically 
      based rejection of in vitro-expanded human neural cells confounds their use as 
      donor cells. Here, we demonstrate that the expression of human leukocyte antigen 
      (HLA) class I and II molecules, but not the co-stimulatory proteins CD40, CD80 
      and CD86, substantially increase during expansion of neurospheres. Furthermore, 
      peripheral lymphocytes were unresponsive when co-cultured with in vitro-expanded 
      neural cells. Taken together, these results suggest a low immunogenicity of these 
      cultured human neural cells despite HLA incompatibility and high HLA expression.
FAU - Odeberg, Jenny
AU  - Odeberg J
AD  - Neurotec Department, Division of Experimental Geriatrics, Karolinska Institutet, 
      Karolinska University Hospital, KFC, 4th floor, Novum, SE-141 86 Stockholm, 
      Sweden.
FAU - Piao, Jing-Hua
AU  - Piao JH
FAU - Samuelsson, Eva-Britt
AU  - Samuelsson EB
FAU - Falci, Scott
AU  - Falci S
FAU - Akesson, Elisabet
AU  - Akesson E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Analysis of Variance
MH  - Antigens, CD/metabolism
MH  - Cell Count/methods
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques/methods
MH  - Embryo, Mammalian
MH  - Female
MH  - Fetus
MH  - Flow Cytometry/methods
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Lymphocyte Activation/physiology
MH  - Major Histocompatibility Complex/*physiology
MH  - Neurons/*immunology
MH  - Pregnancy
MH  - Prosencephalon/*cytology/embryology
MH  - Spinal Cord/*cytology/embryology
MH  - Stem Cells/metabolism
MH  - Time Factors
EDAT- 2005/03/08 09:00
MHDA- 2005/05/28 09:00
CRDT- 2005/03/08 09:00
PHST- 2004/05/19 00:00 [received]
PHST- 2004/09/09 00:00 [revised]
PHST- 2004/11/24 00:00 [accepted]
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/05/28 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - S0165-5728(04)00447-3 [pii]
AID - 10.1016/j.jneuroim.2004.11.016 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2005 Apr;161(1-2):1-11. doi: 10.1016/j.jneuroim.2004.11.016.