PMID- 15749024
OWN - NLM
STAT- MEDLINE
DCOM- 20050728
LR  - 20220129
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 29
IP  - 6
DP  - 2005 Mar 21
TI  - The interleukin-1-related cytokine IL-1F8 is expressed in glial cells, but fails 
      to induce IL-1beta signalling responses.
PG  - 245-50
AB  - The putative new interleukin (IL)-1 family member IL-1F8 (IL-1eta, IL-1H2) has 
      been shown recently to activate mitogen activated protein kinases (MAPKs), 
      extracellular signal-regulated protein kinase (ERK1/2) and c-Jun N-terminal 
      kinase (JNK), and nuclear factor-kappa B (NFkappa B) via a mechanism that 
      requires IL-1Rrp2 expression in cell lines. The aim of this study was to test the 
      hypothesis that IL-1F8 contributes to brain inflammation and injury, by studying 
      its expression and actions in the different cell types of the mouse brain in 
      culture. Messenger RNA for IL-1F8 was detected in neurons and glia (microglial 
      cells, oligodendrocytes progenitor cells and to a lesser extent astrocytes) by 
      RT-PCR. Bacterial lipopolysaccharide (LPS) had no effect on IL-1F8 mRNA levels in 
      mixed glial cultures. Recombinant mouse IL-1beta induced strong activation of 
      ERK1/2, p38, JNK and NFkappa B, and significant release of IL-6 and PGE2, which 
      was blocked by IL-1ra. In contrast, recombinant mouse IL-1F8 did not influence 
      any of these parameters. These results demonstrate that CNS cells may be a source 
      of IL-1F8, but the failure of LPS to modulate IL-1F8 mRNA expression, and of 
      recombinant IL-1F8 to induce any of the classical IL-1 responses, suggest that 
      this cytokine has restricted activities in the brain, or that it may act via 
      alternative pathway(s).
FAU - Wang, Penglian
AU  - Wang P
AD  - School of Biological Sciences, 1-124 Stopford Building, University of Manchester, 
      Oxford Road, Manchester M13 9PT, UK.
FAU - Meinhardt, Brenda
AU  - Meinhardt B
FAU - Andre, Ralph
AU  - Andre R
FAU - Renshaw, Blair R
AU  - Renshaw BR
FAU - Kimber, Ian
AU  - Kimber I
FAU - Rothwell, Nancy J
AU  - Rothwell NJ
FAU - Pinteaux, Emmanuel
AU  - Pinteaux E
LA  - eng
GR  - G9219675/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (IL-1F8 protein, mouse)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Brain/cytology/metabolism
MH  - Cells, Cultured
MH  - Dinoprostone/biosynthesis
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Gene Expression
MH  - Interleukin-1/*biosynthesis/genetics/*pharmacology
MH  - Interleukin-6/biosynthesis
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Neuroglia/drug effects/*immunology
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Signal Transduction
EDAT- 2005/03/08 09:00
MHDA- 2005/07/29 09:00
CRDT- 2005/03/08 09:00
PHST- 2004/06/01 00:00 [received]
PHST- 2004/12/03 00:00 [revised]
PHST- 2004/12/16 00:00 [accepted]
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/07/29 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - S1043-4666(05)00005-0 [pii]
AID - 10.1016/j.cyto.2004.12.002 [doi]
PST - ppublish
SO  - Cytokine. 2005 Mar 21;29(6):245-50. doi: 10.1016/j.cyto.2004.12.002.