PMID- 15749105
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20240328
IS  - 0022-1910 (Print)
IS  - 1879-1611 (Electronic)
IS  - 0022-1910 (Linking)
VI  - 51
IP  - 2
DP  - 2005 Feb
TI  - Comparative analysis of selected genes from Diachasmimorpha longicaudata 
      entomopoxvirus and other poxviruses.
PG  - 207-20
AB  - The Diachasmimorpha longicaudata entomopoxvirus (DlEPV) is the first symbiotic 
      EPV described from a parasitic wasp. The DlEPV is introduced into the tephritid 
      fruit fly larval host along with the wasp egg at oviposition. We sequenced a 
      shotgun genomic library of the DlEPV DNA and analyzed and compared the predicted 
      protein sequences of eight ORFs with those of selected poxviruses and other 
      organisms. BlastP searches showed that five of these are homologous to poxvirus 
      putative proteins such as metalloprotease, a putative membrane protein, late 
      transcription factor-3, virion surface protein, and poly (A) polymerase (PAP) 
      regulatory small subunit. Three of these are similar to those of other organisms 
      such as the gamma-glutamyltransferase (GGT) of Arabidopsis thaliana, eukaryotic 
      initiation factor 4A (eIF4A) of Caenorhabditis briggsae and lambda phage 
      integrase (lambda-Int) of Enterococcus faecium. Transcription motifs for early 
      (TGA,A/T,XXXXA) or late (TAAATG, TAAT, or TAAAT) gene expression conserved in 
      poxviruses were identified with those ORFs. Phylogenetic analysis of multiple 
      alignments of five ORFs and 20 poxvirus homologous sequences and of a concatenate 
      of multiple alignments suggested that DlEPV probably diverged from the ancestral 
      node between the fowlpox virus and the genus B, lepidopteran and orthopteran 
      EPVs, to which Amsacta moorei and Melanoplus sanguinipes EPV, respectively, 
      belong. The DlEPV putative GGT, eIF4A, and lambda-Int contained many conserved 
      domains that typified these proteins. These homologues may be involved in either 
      viral pathogenicity or enhancing parasitism via the gamma-glutamyl cycle and 
      compensation of eIF4A levels in the parasitized fly, or via the integration of a 
      portion of the viral genome into the wasp and/or parasitized fly.
FAU - Hashimoto, Y
AU  - Hashimoto Y
AD  - Department of Entomology and Nematology, University of Florida, Gainesville, FL 
      32611-0620, USA.
FAU - Lawrence, P O
AU  - Lawrence PO
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Insect Physiol
JT  - Journal of insect physiology
JID - 2985080R
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Motifs/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cluster Analysis
MH  - Computational Biology
MH  - Entomopoxvirinae/*genetics
MH  - Genomic Library
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - *Phylogeny
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Viral Proteins/*genetics
MH  - Wasps/*virology
PMC - PMC7094658
EDAT- 2005/03/08 09:00
MHDA- 2005/06/09 09:00
PMCR- 2005/01/08
CRDT- 2005/03/08 09:00
PHST- 2004/10/14 00:00 [received]
PHST- 2004/10/22 00:00 [accepted]
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
PHST- 2005/01/08 00:00 [pmc-release]
AID - S0022-1910(04)00185-4 [pii]
AID - 10.1016/j.jinsphys.2004.10.010 [doi]
PST - ppublish
SO  - J Insect Physiol. 2005 Feb;51(2):207-20. doi: 10.1016/j.jinsphys.2004.10.010.