PMID- 15749106
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20061115
IS  - 0022-1910 (Print)
IS  - 0022-1910 (Linking)
VI  - 51
IP  - 2
DP  - 2005 Feb
TI  - Morphogenesis and cytopathic effects of the Diachasmimorpha longicaudata 
      entomopoxvirus in host haemocytes.
PG  - 221-33
AB  - The Diachasmimorpha longicaudata entomopoxvirus (DlEPV), the first reported 
      symbiotic entomopoxvirus, occurs in the venom apparatus of D. longicaudata female 
      wasps and is introduced into Anastrepha suspensa larvae during parasitism. The 
      DlEPV 250-300 kb double stranded DNA genome encodes putative proteins having 30 
      to >60% amino acid identity with poxvirus homologs such as DNA helicase, 
      DNA-dependent RNA polymerase, and the poxvirus-specific rifampicin resistance 
      protein. Although the molecular characterization of DlEPV is progressing, little 
      is known about its morphogenesis in and effects on host haemocytes. This paper 
      describes (1) haemocytes of third instar A. suspensa, (2) DlEPV infection and 
      morphogenesis, and (3) DlEPV-induced changes in haemocytes. A. suspensa third 
      instars have 3-4 haemocyte morphotypes. Dot blots of DNA from infected haemocytes 
      hybridized with a digoxigenin-labeled DlEPV genomic probe as early as 4 h 
      post-parasitism (hpp) and the intensity of the signal increased with time through 
      40 hpp. Immunofluorescence microscopy localized DlEPV proteins in cytoplasmic 
      (but not nuclear) sites of infected haemocytes, within 24-36 hpp. Electron 
      microscopy confirmed the presence of viral envelopes, immature spheroids with 
      centric nucleoids, budding virus, and extracellular enveloped virus in three 
      haemocyte types, 24-84 hpp and later. Infected haemocytes exhibited blebbing, DNA 
      concatenation, and inability to encapsulate sephadex beads in vitro. These data 
      indicate that DlEPV disrupts the normal function of host haemocytes, thereby 
      insuring the successful development of D. longicaudata offspring and as such 
      should be regarded as a symbiont of the wasp.
FAU - Lawrence, Pauline O
AU  - Lawrence PO
AD  - Department of Entomology and Nematology, University of Florida, 970 Natural Area 
      Drive, Gainesville, FL 32611-0620, USA. pol@ifas.ufl.edu
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Insect Physiol
JT  - Journal of insect physiology
JID - 2985080R
SB  - IM
MH  - Animals
MH  - Entomopoxvirinae/pathogenicity/*physiology/ultrastructure
MH  - Hemocytes/*ultrastructure/virology
MH  - Larva/parasitology/virology
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Molecular Probe Techniques
MH  - *Symbiosis
MH  - Tephritidae/*parasitology/*virology
MH  - Virus Assembly/physiology
MH  - Wasps/*virology
EDAT- 2005/03/08 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/03/08 09:00
PHST- 2004/08/20 00:00 [received]
PHST- 2004/12/08 00:00 [accepted]
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - S0022-1910(04)00211-2 [pii]
AID - 10.1016/j.jinsphys.2004.12.003 [doi]
PST - ppublish
SO  - J Insect Physiol. 2005 Feb;51(2):221-33. doi: 10.1016/j.jinsphys.2004.12.003.