PMID- 15749998
OWN - NLM
STAT- MEDLINE
DCOM- 20050422
LR  - 20161124
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 53
IP  - 3
DP  - 2005 Mar
TI  - Sperm and blastomere aneuploidy detection in reproductive genetics and medicine.
PG  - 261-7
AB  - The use of multiple probes in fluorescence in situ hybridization (FISH) permits 
      the simultaneous analysis of several chromosomes in both blastomeres and 
      spermatozoa. Preimplantation genetic diagnosis (PGD) for aneuploidy provides 
      information on embryonic chromosomal status, enabling the selection of embryos 
      carrying aneuploid condition. This strategy directly affects implantation, as 
      documented for patients with a poor prognosis for pregnancy, who have the 
      tendency to generate high proportions of chromosomally abnormal embryos. PGD for 
      aneuploidy also has contributed information on early phases in human embryology 
      by clarifying the molecular basis in some cases of irregular development. 
      Multicolor FISH has also been used to study chromosomes on spermatozoa. 
      Experimental strategies and modifications enabled the analysis of samples with a 
      very low number of sperm cells, including samples retrieved from the genital 
      tract or directly from the testicular tissue. The results confirmed that the 
      incidence of aneuploidy increases proportionally with the severity of the 
      male-factor condition. This observation suggests that, in selected cases, the 
      paternal contribution to aneuploidy in the developing conceptus could be more 
      relevant than expected from general data from aborted fetuses and live births.
FAU - Gianaroli, Luca
AU  - Gianaroli L
AD  - S.I.S.Me.R., Reproductive Medicine Unit, Via Mazzini, 123 40138 Bologna, Italy. 
      sismer@sismer.it
FAU - Magli, M Cristina
AU  - Magli MC
FAU - Ferraretti, Anna P
AU  - Ferraretti AP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - *Aneuploidy
MH  - Blastomeres/*diagnostic imaging
MH  - Congenital Abnormalities/diagnosis
MH  - Female
MH  - Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Oligospermia/genetics/pathology
MH  - Polyploidy
MH  - Predictive Value of Tests
MH  - *Preimplantation Diagnosis
MH  - Spermatozoa/*ultrastructure
MH  - Ultrasonography
EDAT- 2005/03/08 09:00
MHDA- 2005/04/23 09:00
CRDT- 2005/03/08 09:00
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/04/23 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - 53/3/261 [pii]
AID - 10.1369/jhc.4B6434.2005 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2005 Mar;53(3):261-7. doi: 10.1369/jhc.4B6434.2005.