PMID- 15750010
OWN - NLM
STAT- MEDLINE
DCOM- 20050422
LR  - 20101118
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 53
IP  - 3
DP  - 2005 Mar
TI  - FISH analysis of all fetal nucleated cells in maternal whole blood: improved 
      specificity by the use of two Y-chromosome probes.
PG  - 319-22
AB  - Current cytogenetic approaches in noninvasive prenatal diagnosis focus on fetal 
      nucleated red blood cells in maternal blood. This practice may be too restrictive 
      because a vast proportion of other fetal cells is ignored. Recent studies have 
      indicated that fetal cells can be directly detected, without prior enrichment, in 
      maternal blood samples by fluorescence in situ hybridization (FISH) analysis for 
      chromosomes X and Y (XY-FISH). In our blinded analysis of 40 maternal blood 
      samples, we therefore examined all fetal cells without any enrichment. Initial 
      examinations using conventional XY-FISH indicated a low specificity of 69.4%, 
      which could be improved to 89.5% by the use of two different 
      Y-chromosome-specific probes (YY-FISH) with only a slight concomitant decrease in 
      sensitivity (52.4% vs 42.9%). On average, 12-20 male fetal cells/ml of maternal 
      blood were identified by XY- and YY-FISH, respectively.
FAU - Mergenthaler, Susanne
AU  - Mergenthaler S
AD  - Laboratory for Prenatal Medicine, University Women's Hospital, Spitalstr. 21, 
      4031 Basel, Switzerland.
FAU - Babochkina, Tatiana
AU  - Babochkina T
FAU - Kiefer, Vivian
AU  - Kiefer V
FAU - Lapaire, Olaf
AU  - Lapaire O
FAU - Holzgreve, Wolfgang
AU  - Holzgreve W
FAU - Hahn, Sinuhe
AU  - Hahn S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
SB  - IM
MH  - Cell Count
MH  - Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - False Positive Reactions
MH  - Female
MH  - Fetal Blood/cytology
MH  - Fetus/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy/*blood
MH  - Sensitivity and Specificity
MH  - Sex Determination Analysis
EDAT- 2005/03/08 09:00
MHDA- 2005/04/23 09:00
CRDT- 2005/03/08 09:00
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/04/23 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - 53/3/319 [pii]
AID - 10.1369/jhc.4A6404.2005 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2005 Mar;53(3):319-22. doi: 10.1369/jhc.4A6404.2005.