PMID- 15750029
OWN - NLM
STAT- MEDLINE
DCOM- 20050422
LR  - 20220316
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 53
IP  - 3
DP  - 2005 Mar
TI  - An approach for quantitative assessment of fluorescence in situ hybridization 
      (FISH) signals for applied human molecular cytogenetics.
PG  - 401-8
AB  - A number of applied molecular cytogenetic studies require the quantitative 
      assessment of fluorescence in situ hybridization (FISH) signals (for example, 
      interphase FISH analysis of aneuploidy by chromosome enumeration DNA probes; 
      analysis of somatic pairing of homologous chromosomes in interphase nuclei; 
      identification of chromosomal heteromorphism after FISH with satellite DNA probes 
      for differentiation of parental origin of homologous chromosome, etc.). We have 
      performed a pilot study to develop a simple technique for quantitative assessment 
      of FISH signals by means of the digital capturing of microscopic images and the 
      intensity measuring of hybridization signals using Scion Image software, commonly 
      used for quantification of electrophoresis gels. We have tested this approach by 
      quantitative analysis of FISH signals after application of chromosome-specific 
      DNA probes for aneuploidy scoring in interphase nuclei in cells of different 
      human tissues. This approach allowed us to exclude or confirm a low-level mosaic 
      form of aneuploidy by quantification of FISH signals (for example, discrimination 
      of pseudo-monosomy and artifact signals due to over-position of hybridization 
      signals). Quantification of FISH signals was also used for analysis of somatic 
      pairing of homologous chromosomes in nuclei of postmortem brain tissues after 
      FISH with "classical" satellite DNA probes for chromosomes 1, 9, and 16. This 
      approach has shown a relatively high efficiency for the quantitative registration 
      of chromosomal heteromorphism due to variations of centromeric alphoid DNA in 
      homologous parental chromosomes. We propose this approach to be efficient and to 
      be considered as a useful tool in addition to visual FISH signal analysis for 
      applied molecular cytogenetic studies.
FAU - Iourov, Ivan Y
AU  - Iourov IY
AD  - National Center of Mental Health, Russian Academy of Medical Sciences, Zagorodnoe 
      sh.2, 119152 Moscow, Russia.
FAU - Soloviev, Ilia V
AU  - Soloviev IV
FAU - Vorsanova, Svetlana G
AU  - Vorsanova SG
FAU - Monakhov, Viktor V
AU  - Monakhov VV
FAU - Yurov, Yuri B
AU  - Yurov YB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (DNA Probes)
SB  - IM
MH  - Aneuploidy
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, X
MH  - DNA Probes
MH  - Down Syndrome/genetics
MH  - Female
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Interphase
MH  - Male
MH  - Mosaicism
MH  - Nondisjunction, Genetic
MH  - Pilot Projects
MH  - Reproducibility of Results
MH  - Software
EDAT- 2005/03/08 09:00
MHDA- 2005/04/23 09:00
CRDT- 2005/03/08 09:00
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/04/23 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - 53/3/401 [pii]
AID - 10.1369/jhc.4A6419.2005 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2005 Mar;53(3):401-8. doi: 10.1369/jhc.4A6419.2005.