PMID- 15750169
OWN - NLM
STAT- MEDLINE
DCOM- 20050503
LR  - 20191210
IS  - 0161-5505 (Print)
IS  - 0161-5505 (Linking)
VI  - 46
IP  - 3
DP  - 2005 Mar
TI  - Dedicated pinhole SPECT of intestinal neutrophil recruitment in a mouse model of 
      dextran sulfate sodium-induced colitis.
PG  - 526-31
AB  - Evaluating the efficacy of therapy in experimental inflammatory bowel disease 
      (IBD) requires information about inflammatory activity in bowel segments or 
      leukocyte recruitment and about kinetics in the follow-up of treatment. This 
      study evaluated a noninvasive scintigraphic technique able to assess neutrophil 
      trafficking in a mouse model of dextran sulfate sodium (DSS)-induced colitis. 
      METHODS: Groups of 4 BALB/c mice were assessed at baseline and after 1, 3, 5, and 
      8 d of treatment with DSS. Donor neutrophils were harvested by rinsing of the 
      peritoneal cavity with phosphate-buffered saline 5 h after intraperitoneal 
      injection of proteose peptone contained in phosphate-buffered saline and labeled 
      with freshly prepared (99m)Tc-hexamethylpropylene amine oxime (HMPAO). Pinhole 
      SPECT of the abdomen was performed 1 h after reinjection of 50 MBq of labeled 
      neutrophils. In addition, the severity of inflammation was determined by 
      histologic examination. The possibilities of the technique were illustrated by 
      scintigraphic assessment of neutrophil trafficking with and without blocking of 
      neutrophil migration by a CD97 monoclonal antibody in mice with DSS-induced 
      colitis. RESULTS: Colonic uptake of (99m)Tc-HMPAO neutrophils was determined with 
      dedicated animal pinhole SPECT in mice with DSS-induced colitis and correlated 
      well with histologic findings (R = 0.81) and wet colon weight (R = 0.87) and 
      moderately with clinical weight loss (R = 0.62). The neutrophil uptake ratio was 
      reduced significantly (P < 0.01) by blocking of neutrophil migration capacity 
      with the CD97 antibody. CONCLUSION: Animal pinhole SPECT can be used to study 
      inflammatory activity and neutrophil recruitment in vivo in experimental colitis.
FAU - Bennink, Roelof J
AU  - Bennink RJ
AD  - Department of Nuclear Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands. r.bennink@amc.uva.nl
FAU - Hamann, Jorg
AU  - Hamann J
FAU - de Bruin, Kora
AU  - de Bruin K
FAU - ten Kate, Fiebo J W
AU  - ten Kate FJ
FAU - van Deventer, Sander J H
AU  - van Deventer SJ
FAU - te Velde, Anje A
AU  - te Velde AA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - J Nucl Med
JT  - Journal of nuclear medicine : official publication, Society of Nuclear Medicine
JID - 0217410
RN  - 0 (Radiopharmaceuticals)
RN  - 3B744AG22N (Technetium Tc 99m Exametazime)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Colitis/chemically induced/*diagnostic imaging/*metabolism/pathology
MH  - Dextran Sulfate
MH  - Disease Models, Animal
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neutrophils/*diagnostic imaging/*metabolism
MH  - Radiopharmaceuticals/pharmacokinetics
MH  - Technetium Tc 99m Exametazime/*pharmacokinetics
MH  - Tomography, Emission-Computed, Single-Photon/*methods
EDAT- 2005/03/08 09:00
MHDA- 2005/05/04 09:00
CRDT- 2005/03/08 09:00
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/05/04 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - 46/3/526 [pii]
PST - ppublish
SO  - J Nucl Med. 2005 Mar;46(3):526-31.