PMID- 15750849
OWN - NLM
STAT- MEDLINE
DCOM- 20051114
LR  - 20181113
IS  - 0142-4319 (Print)
IS  - 0142-4319 (Linking)
VI  - 25
IP  - 8
DP  - 2004
TI  - Involvement of c-Jun N-terminal kinase activities in skeletal muscle 
      differentiation.
PG  - 645-55
AB  - Previous studies on skeletal muscle differentiation showed that myogenesis is 
      regulated by extracellular signal-regulated kinases (ERK-1/-2) and p38 mitogen 
      activated kinase (MAPK) pathways. Present study shows that c-Jun NH2-terminal 
      protein kinase (JNK) activities were up regulated during skeletal muscle 
      differentiation in rat skeletal muscle L6E9 cells, as determined by Western 
      immunoblot of differentiating cells probed with anti-phospho-JNK antibody. 
      Inhibition of JNK activities by JNK inhibitor II drastically inhibited 
      differentiation as determined by decreased myosin, myogenin expression and 
      creatine kinase activity. The inhibition of the differentiation was regulated by 
      apoptosis as determined by the detection of poly(ADP-ribose) polymerase (PARP) 
      cleavage, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) positive cells when JNK activities were inhibited. Apoptosis was 
      accompanied by marked expression and activation of c-Jun and p53 transcription 
      factors. Taken together, our results indicate that basal JNK activities are 
      essential for regulating skeletal muscle differentiation, and inhibition of JNK 
      activation affects myogenesis by apoptosis dependent on c-Jun and p53 
      transcription factors.
FAU - Khurana, Ashwani
AU  - Khurana A
AD  - Signal Transduction Research Laboratory, Department of Biotechnology, National 
      Institute of Pharmaceutical Education and Research, Sector 67, S.A.S NAGAR, 
      Punjab 160062, India.
FAU - Dey, Chinmoy S
AU  - Dey CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050224
PL  - Netherlands
TA  - J Muscle Res Cell Motil
JT  - Journal of muscle research and cell motility
JID - 8006298
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Blotting, Western
MH  - Cell Differentiation/physiology
MH  - Cell Line
MH  - Cell Survival/physiology
MH  - Creatine Kinase/biosynthesis
MH  - Enzyme Activation
MH  - In Situ Nick-End Labeling
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Microscopy, Fluorescence
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Muscle, Skeletal/*cytology/*enzymology
MH  - Rats
MH  - Tetrazolium Salts/metabolism
MH  - Thiazoles/metabolism
MH  - Tumor Suppressor Protein p53/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2005/03/08 09:00
MHDA- 2005/11/15 09:00
CRDT- 2005/03/08 09:00
PHST- 2004/08/19 00:00 [received]
PHST- 2004/11/25 00:00 [accepted]
PHST- 2005/03/08 09:00 [pubmed]
PHST- 2005/11/15 09:00 [medline]
PHST- 2005/03/08 09:00 [entrez]
AID - 10.1007/s10974-004-7099-1 [doi]
PST - ppublish
SO  - J Muscle Res Cell Motil. 2004;25(8):645-55. doi: 10.1007/s10974-004-7099-1. Epub 
      2005 Feb 24.