PMID- 15752135
OWN - NLM
STAT- MEDLINE
DCOM- 20050817
LR  - 20061115
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 6
IP  - 4
DP  - 2005 Apr
TI  - The cellular pathway of CD1e in immature and maturing dendritic cells.
PG  - 286-302
AB  - Dendritic cells (DCs) present antigens to T cells via CD1, HLA class I or class 
      II molecules. During maturation, HLA class II-restricted presentation is 
      optimized. The relocalization of CD1e from Golgi to endosomal compartments during 
      DC maturation suggests also an optimization of the antigen-presentation pathway 
      via CD1 molecules. We here detail the biosynthesis and cellular pathway of CD1e 
      in immature and maturing DCs. Unlike the other CD1 molecules, CD1e was found to 
      reach late endosomes through sorting endosomes, without passing through the 
      plasma membrane in either immature or maturing cells. After induction of DC 
      maturation, CD1e disappeared rapidly from the Golgi and was transiently localized 
      in HLA-DR+ vesicles, while the number of CD1e+/CD1b+ compartments increased for 
      at least 20 h. High-resolution light microscopy showed that, in immature DCs, 
      CD1e+ vesicles were often in close apposition to EEA1+ or HLA-DR+ compartments, 
      while CD1e displayed a nearly exclusive distribution in the lysosomes of mature 
      DCs, a finding corroborated by immunoelectron microscopy. During maturation, CD1e 
      synthesis progressively declined, while the endosomal cleavage of CD1e still 
      occurred. Thus, CD1e displays peculiar properties, suggesting an unexpected role 
      among the family of CD1 antigen-presenting molecules.
FAU - Angenieux, Catherine
AU  - Angenieux C
AD  - INSERM E 0345, Etablissement Francais du Sang-Alsace, 67065 Strasbourg, France.
FAU - Fraisier, Vincent
AU  - Fraisier V
FAU - Maitre, Blandine
AU  - Maitre B
FAU - Racine, Victor
AU  - Racine V
FAU - van der Wel, Nicole
AU  - van der Wel N
FAU - Fricker, Dominique
AU  - Fricker D
FAU - Proamer, Fabienne
AU  - Proamer F
FAU - Sachse, Martin
AU  - Sachse M
FAU - Cazenave, Jean-Pierre
AU  - Cazenave JP
FAU - Peters, Peter
AU  - Peters P
FAU - Goud, Bruno
AU  - Goud B
FAU - Hanau, Daniel
AU  - Hanau D
FAU - Sibarita, Jean-Baptiste
AU  - Sibarita JB
FAU - Salamero, Jean
AU  - Salamero J
FAU - de la Salle, Henri
AU  - de la Salle H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1b antigen)
RN  - 0 (CD1e antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Protease Inhibitors)
SB  - IM
MH  - Antigens, CD1/*metabolism
MH  - *Cell Differentiation
MH  - Cell Membrane/metabolism
MH  - Dendritic Cells/*cytology/drug effects/*metabolism
MH  - Endosomes/metabolism
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Humans
MH  - Microscopy, Immunoelectron
MH  - Protease Inhibitors/pharmacology
MH  - Protein Transport
EDAT- 2005/03/09 09:00
MHDA- 2005/08/18 09:00
CRDT- 2005/03/09 09:00
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/08/18 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - TRA272 [pii]
AID - 10.1111/j.1600-0854.2005.00272.x [doi]
PST - ppublish
SO  - Traffic. 2005 Apr;6(4):286-302. doi: 10.1111/j.1600-0854.2005.00272.x.