PMID- 15752702
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20191210
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1748
IP  - 1
DP  - 2005 Apr 15
TI  - The target of RNAIII-activating protein (TRAP) from Staphylococcus aureus: 
      purification, crystallization and preliminary X-ray analysis.
PG  - 134-6
AB  - The target of the RNAIII-activating protein (TRAP) is a 21 kDa protein in which 
      phosphorylation is activated by the RNAIII-activating protein (RAP), which causes 
      an increase in RNAII and RNAIII synthesis and the production of the virulence 
      factors. In an attempt to examine the structural role of TRAP in the signal 
      transduction pathway, TRAP from Staphylococcus aureus was overexpressed, purified 
      and crystallized using PEG 8000 and 5% Jeffamine M600 (pH 7.0), as precipitants 
      by hanging-drop vapour diffusion methods at 287 K. The crystals belong to the 
      orthorhombic space group, P2(1)2(1)2(1), with unit cell parameters of a=39.68, 
      b=50.41, c=85.45 A. There is one monomer of TRAP per crystallographic asymmetric 
      unit with a crystal volume per protein mass (V(M)) of 2.06 A(3) Da(-1) and a 
      solvent content of 40.3%. A complete data set diffracting to 1.9 A resolution was 
      collected from a single crystal at 100 K using a synchrotron-radiation source.
FAU - Han, Young-Hyun
AU  - Han YH
AD  - Department of Molecular Cell Biology, Samsung Biomedical Research Institute, 
      Sungkyunkwan University School of Medicine, 300 Chunchundong, Suwon 440-746, 
      Korea.
FAU - Kim, Yang-Gyun
AU  - Kim YG
FAU - Kim, Dong Young
AU  - Kim DY
FAU - Ha, Sung Chul
AU  - Ha SC
FAU - Lokanath, Neratur K
AU  - Lokanath NK
FAU - Kim, Kyeong Kyu
AU  - Kim KK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050116
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RAP protein, Staphylococcus aureus)
RN  - 0 (TRAP protein, Staphylococcus)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Bacterial Proteins/*chemistry/genetics/*metabolism
MH  - Carrier Proteins/*metabolism
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Phosphoproteins/*chemistry/genetics/*metabolism
MH  - Signal Transduction/physiology
EDAT- 2005/03/09 09:00
MHDA- 2005/04/29 09:00
CRDT- 2005/03/09 09:00
PHST- 2004/06/22 00:00 [received]
PHST- 2004/11/24 00:00 [revised]
PHST- 2004/11/24 00:00 [accepted]
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - S1570-9639(04)00333-4 [pii]
AID - 10.1016/j.bbapap.2004.11.021 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2005 Apr 15;1748(1):134-6. doi: 
      10.1016/j.bbapap.2004.11.021. Epub 2005 Jan 16.