PMID- 15752724
OWN - NLM
STAT- MEDLINE
DCOM- 20050517
LR  - 20210524
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 436
IP  - 1
DP  - 2005 Apr 1
TI  - Cathepsin B promotes both motility and invasiveness of oral carcinoma cells.
PG  - 187-95
AB  - We previously demonstrated that overexpression of cathepsin B (CB) protease in 
      oral squamous cell carcinomas correlated positively with advanced tumor stage and 
      poor histologic malignancy grade. Here we examined whether CB contributes to the 
      invasiveness of oral carcinoma cells. For RNA-mediated inhibition, two ribozymes 
      that target CB mRNA were designed and stably expressed in the oral squamous cell 
      carcinoma cell line 1386Tu. Both ribozymes diminished expression of CB mRNA, 
      protein, and activity, without affecting cathepsin D or beta-actin, as determined 
      by quantitative real-time PCR, Western blots, and protease activity assays. 
      Matrigel invasion assays showed that the invasiveness of the cells was 
      significantly reduced by the expressed ribozymes and, surprisingly, the 
      motilities of the ribozyme-transfected cells were also diminished. Our results 
      document a direct role for CB in promoting oral cancer spread and invasion, and 
      open the possibility of controlling oral carcinoma malignancy and metastasis by 
      targeting CB with RNA inhibitor strategies.
FAU - Wickramasinghe, Nalinie S
AU  - Wickramasinghe NS
AD  - Department of Medicine, James Graham Brown Cancer Center, University of 
      Louisville, Louisville, KY 40202, USA.
FAU - Nagaraj, Nagathihalli S
AU  - Nagaraj NS
FAU - Vigneswaran, Nadarajah
AU  - Vigneswaran N
FAU - Zacharias, Wolfgang
AU  - Zacharias W
LA  - eng
GR  - R01 DE013150/DE/NIDCR NIH HHS/United States
GR  - DE13150/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Biomarkers)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Catalytic)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.22.1 (Cathepsin B)
SB  - IM
MH  - Base Sequence
MH  - Biomarkers
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Cathepsin B/*physiology
MH  - Cell Movement/*physiology
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mouth Neoplasms/*pathology
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Peptide Hydrolases/metabolism
MH  - Prognosis
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - RNA, Catalytic/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2005/03/09 09:00
MHDA- 2005/05/18 09:00
CRDT- 2005/03/09 09:00
PHST- 2005/01/06 00:00 [received]
PHST- 2005/01/27 00:00 [revised]
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/05/18 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - S0003-9861(05)00053-6 [pii]
AID - 10.1016/j.abb.2005.01.023 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2005 Apr 1;436(1):187-95. doi: 10.1016/j.abb.2005.01.023.