PMID- 15753572
OWN - NLM
STAT- MEDLINE
DCOM- 20051027
LR  - 20061115
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 109
IP  - 1-3
DP  - 2005
TI  - Minichromosomes derived from the B chromosome of maize.
PG  - 156-65
AB  - Fourteen minichromosomes derived from the B chromosome of maize are described. 
      The centromeric region of the B chromosome contains a specific repetitive DNA 
      element called the B repeat. This sequence was used to determine the transmission 
      frequency of the different types of minichromosomes over several generations via 
      Southern blot analysis at each generation. In general, the minichromosomes have 
      transmission rates below the theoretical 50% frequency of a univalent chromosome. 
      The gross structure of each minichromosome was determined using fluorescence in 
      situ hybridization (FISH) on root tip chromosome spreads. The presence of the B 
      centromeric repeat and of the adjacent heterochromatic knob sequences was 
      determined for each minichromosome. In two cases, the amount of the centromeric 
      knob repeat is increased relative to the progenitor chromosome. Other isolates 
      have reduced or undetectable levels of the knob sequence. Potential uses of the 
      minichromosomes are discussed.
CI  - Copyright 2005 S. Karger AG, Basel.
FAU - Kato, A
AU  - Kato A
AD  - Division of Biological Sciences, University of Missouri, Columbia, MO 65211-7400, 
      USA.
FAU - Zheng, Y-Z
AU  - Zheng YZ
FAU - Auger, D L
AU  - Auger DL
FAU - Phelps-Durr, T
AU  - Phelps-Durr T
FAU - Bauer, M J
AU  - Bauer MJ
FAU - Lamb, J C
AU  - Lamb JC
FAU - Birchler, J A
AU  - Birchler JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Cell Nucleus/genetics/ultrastructure
MH  - Chromosome Mapping
MH  - Chromosomes, Plant/genetics/*ultrastructure
MH  - In Situ Hybridization
MH  - Mitosis
MH  - Zea mays/cytology/*genetics
EDAT- 2005/03/09 09:00
MHDA- 2005/10/28 09:00
CRDT- 2005/03/09 09:00
PHST- 2003/10/20 00:00 [received]
PHST- 2004/02/20 00:00 [accepted]
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/10/28 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - 82395 [pii]
AID - 10.1159/000082395 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2005;109(1-3):156-65. doi: 10.1159/000082395.