PMID- 15753598
OWN - NLM
STAT- MEDLINE
DCOM- 20051027
LR  - 20081121
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 109
IP  - 1-3
DP  - 2005
TI  - Development and characterization of potato-Solanum brevidens chromosomal 
      addition/substitution lines.
PG  - 368-72
AB  - Solanum brevidens is a wild diploid potato species possessing high levels of 
      resistances to several major potato diseases. We previously developed fertile 
      somatic hybrids between S. brevidens and the cultivated potato (Solanum 
      tuberosum) in order to introgress disease resistances from this wild species into 
      potato. A series of backcross progenies was developed from a hexaploid somatic 
      hybrid A206. Using a combination of S. brevidens-specific randomly amplified 
      polymorphic DNA (RAPD) markers and a sequential genomic in situ hybridization 
      (GISH) and fluorescence in situ hybridization (FISH) technique, we identified all 
      12 S. brevidens chromosomes in the backcross progenies. Seven potato-S. brevidens 
      monosomic chromosome addition lines (chromosomes 1, 3, 4, 5, 8, 9 and 10) and one 
      monosomic substitution line (chromosome 6) were identified, and the remaining 
      four S. brevidens chromosomes (2, 7, 11, and 12) were included in two other 
      lines. These chromosomal addition/substitution stocks provide valuable tools for 
      potato cytogenetic research, and can be used to introgress disease resistances 
      from S. brevidens into potato.
CI  - Copyright 2005 S. Karger AG, Basel.
FAU - Dong, F
AU  - Dong F
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, 
      USA.
FAU - Tek, A L
AU  - Tek AL
FAU - Frasca, A B L
AU  - Frasca AB
FAU - McGrath, J M
AU  - McGrath JM
FAU - Wielgus, S M
AU  - Wielgus SM
FAU - Helgeson, J P
AU  - Helgeson JP
FAU - Jiang, J
AU  - Jiang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - Genetic Markers
MH  - Immunity, Innate/genetics
MH  - In Situ Hybridization/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Diseases
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Solanum tuberosum/*genetics
EDAT- 2005/03/09 09:00
MHDA- 2005/10/28 09:00
CRDT- 2005/03/09 09:00
PHST- 2003/10/23 00:00 [received]
PHST- 2004/02/25 00:00 [accepted]
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/10/28 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - 82421 [pii]
AID - 10.1159/000082421 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2005;109(1-3):368-72. doi: 10.1159/000082421.