PMID- 15753654
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20210102
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 4
IP  - 3
DP  - 2005 Mar
TI  - High efficiency transduction of dendritic cells by adenoviral vectors targeted to 
      DC-SIGN.
PG  - 289-94
AB  - Dendritic cells (DCs) are a central element in the development of 
      antigen-specific immune responses. The lack of a specific and efficient technique 
      for the in vivo delivery of antigens to DCs remains a major obstacle limiting a 
      vaccine's ability to induce an effective immune response. The efficacy of 
      adenoviral (Ad) vectors in this regard can be enhanced through alterations in 
      vector tropism such that DC-targeted transduction is achieved. Here, the 
      efficiency of DC transduction by Ad vectors retargeted to DC-specific ICAM-3 
      grabbing nonintegrin (DC-SIGN) was studied and compared to that of Ad vectors 
      retargeted through CD40. A comparable and significant enhancement of gene 
      transfer to monocyte derived DCs (MDDCs) was accomplished by means of an Ad 
      vector harboring the Fc-binding domain of Staphylococcus aureus protein A in 
      combination with antibodies to DC-SIGN or to CD40 or with fused complexes of 
      human Ig-Fc with their natural ligands, i.e., ICAM-3 or CD40L, respectively. 
      Whereas CD40-targeted Ad transduction resulted in a more profound phenotypic DC 
      maturation, DC-SIGN- and CD40-targeted Ad both induced similar levels of IL-12 
      secretion. These data demonstrate the usefulness of DC-SIGN as a DC-restricted 
      targeting motif for Ad-mediated vaccination strategies.
FAU - Korokhov, Nikolay
AU  - Korokhov N
AD  - Vector Logics, Inc., Birmingham, Alabama 35233, USA. nkorokhov@vectorlogics.com
FAU - de Gruijl, Tanja D
AU  - de Gruijl TD
FAU - Aldrich, Wayne A
AU  - Aldrich WA
FAU - Triozzi, Pierre L
AU  - Triozzi PL
FAU - Banerjee, Papia T
AU  - Banerjee PT
FAU - Gillies, Stephen D
AU  - Gillies SD
FAU - Curiel, Tyler J
AU  - Curiel TJ
FAU - Douglas, Joanne T
AU  - Douglas JT
FAU - Scheper, Rik J
AU  - Scheper RJ
FAU - Curiel, David T
AU  - Curiel DT
LA  - eng
GR  - R01 CA 86811/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20050320
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (CD40 Antigens)
RN  - 0 (Cancer Vaccines)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - CD40 Antigens/genetics
MH  - Cancer Vaccines/genetics
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Line
MH  - Dendritic Cells/*immunology
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - Immunotherapy, Adoptive
MH  - Lectins, C-Type/*metabolism
MH  - Monocytes/immunology
MH  - Receptors, Cell Surface/*metabolism
MH  - Transduction, Genetic/*methods
EDAT- 2005/03/09 09:00
MHDA- 2006/03/22 09:00
CRDT- 2005/03/09 09:00
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - 1499 [pii]
AID - 10.4161/cbt.4.3.1499 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2005 Mar;4(3):289-94. doi: 10.4161/cbt.4.3.1499. Epub 2005 Mar 
      20.