PMID- 15754381
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20091119
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 45
IP  - 2-3
DP  - 2005 Mar-Apr
TI  - Cross-species sperm-FISH assays for chemical testing and assessing paternal risk 
      for chromosomally abnormal pregnancies.
PG  - 271-83
AB  - The father, like the mother, can transmit genetic defects to his offspring that 
      are detrimental for normal development and a healthy life. Epidemiological 
      studies have identified associations between several paternal exposures and 
      abnormal reproductive outcomes, but these types of studies are inherently complex 
      and expensive, and the risk factors for the paternal contribution to abnormal 
      reproductive outcomes remain poorly understood. Several sensitive methods have 
      been developed for detecting mutations and chromosomal damage directly in sperm. 
      These assays are potential bioindicators for paternal risk factors for 
      infertility, spontaneous abortions, aneuploidy syndromes, and genetic diseases in 
      children. Among these methods, fluorescence in situ hybridization (FISH) has been 
      adapted for the detection of numerical and structural chromosomal abnormalities 
      in the sperm of an expanding number of species, including humans and rodents. 
      Sperm FISH has identified several potential paternal risk factors such as age, 
      drugs, lifestyles, and various environmental/occupational exposures. Here, we 
      summarize the status of the development and usage of these sperm-FISH assays and 
      suggest strategies for prioritizing chemical agents for epidemiological 
      investigations to assess paternal risk for abnormal reproductive outcome.
FAU - Wyrobek, Andrew J
AU  - Wyrobek AJ
AD  - Biosciences Directorate, Lawrence Livermore National Laboratory, University of 
      California, Livermore, California 94550, USA. wyrobek1@llnl.gov
FAU - Schmid, Thomas E
AU  - Schmid TE
FAU - Marchetti, Francesco
AU  - Marchetti F
LA  - eng
GR  - ES 09117-03/ES/NIEHS NIH HHS/United States
GR  - Y1 ES 8016-5/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Mutagens)
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Mice
MH  - Molecular Epidemiology/*methods
MH  - Mutagens
MH  - *Paternal Exposure
MH  - Species Specificity
MH  - *Spermatozoa
RF  - 69
EDAT- 2005/03/09 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/03/09 09:00
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
AID - 10.1002/em.20121 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2005 Mar-Apr;45(2-3):271-83. doi: 10.1002/em.20121.