PMID- 15754420
OWN - NLM
STAT- MEDLINE
DCOM- 20050722
LR  - 20181113
IS  - 1673-1581 (Print)
IS  - 1673-1581 (Linking)
VI  - 6
IP  - 4
DP  - 2005 Apr
TI  - Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit 
      heart to improve heart function.
PG  - 242-8
AB  - OBJECTIVE: The present study was designed to test whether transplantation of 
      human bone marrow-derived mesenchymal stem cells (hMSCs) in New Zealand rabbits 
      with myocardial infarction can improve heart function; and whether engrafted 
      donor cells can survive and transdifferentiated into cardiomyocytes. METHODS: 
      Twenty milliliters bone marrow was obtained from healthy men by bone biopsy. A 
      gradient centrifugation method was used to separate bone marrow cells (BMCs) and 
      red blood cells. BMCs were incubated for 48 h and then washed with 
      phosphate-buffered saline (PBS). The culture medium was changed twice a week for 
      28 d. Finally, hematopoietic cells were washed away to leave only MSCs. Human 
      MSCs (hMSCs) were premarked by BrdU 72 h before the transplantation. Thirty-four 
      New Zealand rabbits were randomly divided into myocardial infarction (MI) control 
      group and cell treated group, which received hMSCs (MI+MSCs) through 
      intramyocardial injection, while the control group received the same volume of 
      PBS. Myocardial infarction was induced by ligation of the left coronary artery. 
      Cell treated rabbits were treated with 5 x 10(6) MSCs transplanted into the 
      infarcted region after ligation of the coronary artery for 1 h, and the control 
      group received the same volume of PBS. Cyclosporin A (oral solution; 10 mg/kg) 
      was provided alone, 24 h before surgery and once a day after MI for 4 weeks. 
      Echocardiography was measured in each group before the surgery and 4 weeks after 
      the surgery to test heart function change. The hearts were harvested for HE 
      staining and immunohistochemical studies after MI and cell transplantation for 4 
      weeks. RESULTS: Our data showed that cardiac function was significantly improved 
      by hMSC transplantation in rabbit infarcted hearts 4 weeks after MI (ejection 
      fraction: 0.695+/-0.038 in the cell treated group (n=12) versus 0.554+/-0.065 in 
      the control group (n=13) (P<0.05). Surviving hMSCs were identified by BrdU 
      positive spots in infarcted region and transdifferentiated into cardiomyocytes 
      characterized with a positive cardiac phenotype: troponin I. CONCLUSION: 
      Transplantation of hMSCs could transdifferentiate into cardiomyocytes and 
      regenerate vascular structures, contributing to functional improvement.
FAU - Wang, Jian-an
AU  - Wang JA
AD  - Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang 
      University, Hangzhou 310016, China.
FAU - Fan, You-qi
AU  - Fan YQ
FAU - Li, Chang-ling
AU  - Li CL
FAU - He, Hong
AU  - He H
FAU - Sun, Yong
AU  - Sun Y
FAU - Lv, Bin-jian
AU  - Lv BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Zhejiang Univ Sci B
JT  - Journal of Zhejiang University. Science. B
JID - 101236535
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Bone Marrow Cells/*cytology
MH  - Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Myocardial Infarction/*physiopathology/*surgery
MH  - Rabbits
MH  - Survival Rate
MH  - Time Factors
PMC - PMC1389731
EDAT- 2005/03/09 09:00
MHDA- 2005/07/23 09:00
PMCR- 2005/04/01
CRDT- 2005/03/09 09:00
PHST- 2005/03/09 09:00 [pubmed]
PHST- 2005/07/23 09:00 [medline]
PHST- 2005/03/09 09:00 [entrez]
PHST- 2005/04/01 00:00 [pmc-release]
AID - 10.1631/jzus.2005.B0242 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci B. 2005 Apr;6(4):242-8. doi: 10.1631/jzus.2005.B0242.