PMID- 15756435
OWN - NLM
STAT- MEDLINE
DCOM- 20050826
LR  - 20141120
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 13
IP  - 4
DP  - 2005 Apr
TI  - Detection of Her2/neu, c-MYC and ZNF217 gene amplification during breast cancer 
      progression using fluorescence in situ hybridization.
PG  - 633-41
AB  - The deletion of tumor suppressor genes and amplification of activating oncogenes 
      appear to be critical events in tumorigenesis. We carried out fluorescence in 
      situ hybridization (FISH) analysis of the breast cancer cell line MCF7 and 
      clinically obtained cancer tissue sections on the basis of which we suggest a 
      breast cancer development model. We selected 28 genes for FISH probes from breast 
      cancer patients. Of the 28 genes studied, 14 were shown to be consistently 
      amplified in the breast cancer cell line MCF7. We selected three genes from 
      clinical tumor samples on the basis of results from MCF7 analysis. The 
      amplification of Her2/neu or ZNF217 is closely associated with the stages of 
      breast cancer. The frequency of amplification of Her2/neu increased notably in 
      patients at stages later than IIB based on TNM staging, whereas the amplification 
      of ZNF217 correlated with T2N1M0 at stage IIB and later stages. c-MYC 
      amplification was not related to the stage. Her2/neu, ZNF217 and c-MYC were found 
      to have a significantly increased copy number in breast cancer cells. In breast 
      cancer progression, c-MYC amplification is an early event, while ZNF217 and 
      Her2/neu amplification may play a role in the later stage of tumor development.
FAU - Shimada, Masayuki
AU  - Shimada M
AD  - Department of Technology Development and Assessment, IDV, SRL Inc, 5-6-50 
      Shinmachi, Hino-shi, Tokyo 191-0002, Japan. komiyams@srl.srl-inc.co.jp
FAU - Imura, Johji
AU  - Imura J
FAU - Kozaki, Takazumi
AU  - Kozaki T
FAU - Fujimori, Takahiro
AU  - Fujimori T
FAU - Asakawa, Shuichi
AU  - Asakawa S
FAU - Shimizu, Nobuyoshi
AU  - Shimizu N
FAU - Kawaguchi, Ryuji
AU  - Kawaguchi R
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Trans-Activators)
RN  - 0 (ZNF217 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Disease Progression
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Tumor Suppressor
MH  - Genes, erbB-2
MH  - Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Proto-Oncogene Proteins c-myc/*biosynthesis/*genetics
MH  - Receptor, ErbB-2/*biosynthesis/*genetics
MH  - Trans-Activators/*biosynthesis/*genetics
EDAT- 2005/03/10 09:00
MHDA- 2005/08/27 09:00
CRDT- 2005/03/10 09:00
PHST- 2005/03/10 09:00 [pubmed]
PHST- 2005/08/27 09:00 [medline]
PHST- 2005/03/10 09:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2005 Apr;13(4):633-41.